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产品描述：人14-3-3蛋白ε(YWHAE)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的YWHAE含量。YWHAE是一个重要靶点。它在细胞信号转导等多种生理过程中扮演关键角色。研究发现其可能与肿瘤发生发展机制相关，通过参与细胞增殖、凋亡等调控过程影响肿瘤生物学行为，有望成为肿瘤治疗等领域的潜在干预靶点。试剂盒检测范围为78 pg/mL-5000 pg/mL，适用于科研场景中探究YWHAE在疾病模型中的表达调控、筛选潜在生物标志物或验证药物干预对靶蛋白的影响；可为细胞分子机制研究、病理模型分析及体外实验验证提供可靠的定量检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：14 3 3 E ELISA Kit; 14 3 3 epsilon ELISA Kit; 14 3 3E ELISA Kit; 14-3-3 protein epsilon ELISA Kit; 14-3-3E ELISA Kit; 1433E_HUMAN ELISA Kit; Epididymis luminal protein 2 ELISA Kit; FLJ45465 ELISA Kit; FLJ53559 ELISA Kit; HEL2 ELISA Kit; KCIP 1 ELISA Kit; KCIP1 ELISA Kit; MDCR ELISA Kit; MDS ELISA Kit; Mitochondrial import stimulation factor L subunit ELISA Kit; Protein kinase C inhibitor protein1 ELISA Kit; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein; epsilon ELISA Kit; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein; epsilon polypeptide ELISA Kit; Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide ELISA Kit; YWHAE ELISA Kit
缩写：YWHAE
Uniprot No.：P62258
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human YWHAE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 86 | |||||
| Range % | 81-92 | ||||||
| 1:2 | Average % | 98 | |||||
| Range % | 92-103 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 88-98 | ||||||
| 1:8 | Average % | 93 | |||||
| Range % | 87-96 | ||||||
回收率：
| The recovery of human YWHAE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 87 | 85-93 | |||||
| EDTA plasma (n=4) | 103 | 98-106 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 50002.5642.6542.6092.438 25001.8971.9451.9211.750 12501.1761.2221.1991.028 6250.7530.7670.7600.589 3120.4600.4540.4570.286 1560.2880.2760.2820.111 780.2260.2310.2290.058 00.1700.1710.171 | |||||||
货期：3-5 working days
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