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产品描述：人泛酰巯基乙胺酶(VNN1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的VNN1含量。VNN1 即Vanin - 1，是一种在多种组织表达的泛酰巯基乙胺酶。其与氧化应激、炎症等生理病理过程关联。研究机制上，它通过调节半胱胺水平影响细胞内氧化还原状态，在代谢性疾病、肿瘤等研究中受关注，为相关疾病防治提供潜在靶点。试剂盒检测范围为78 pg/mL-5000 pg/mL，适用于基础科研领域对VNN1功能的探索性研究，例如在代谢疾病模型或炎症模型中分析VNN1的动态表达规律，评估药物干预或基因调控对VNN1活性的影响，或通过多组学联合分析挖掘其作为潜在生物标志物的价值；为疾病机制研究及药物开发提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：HDLCQ8 ELISA Kit; High density lipoprotein cholesterol level quantitative trait locus 8, included ELISA Kit; Pantetheinase ELISA Kit; Pantetheine hydrolase ELISA Kit; Tiff66 ELISA Kit; V-1 ELISA Kit; Vanin 1 ELISA Kit; Vanin-1 ELISA Kit; Vannin 1 ELISA Kit; Vascular non inflammatory molecule 1 ELISA Kit; Vascular non-inflammatory molecule 1 ELISA Kit; VNN 1 ELISA Kit; VNN1 ELISA Kit; VNN1_HUMAN ELISA Kit
缩写：VNN1
Uniprot No.：O95497
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human pantetheinase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:2 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 95-104 | |||||
回收率：
| The recovery of human pantetheinase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 80-92 | ||||
| EDTA plasma (n=4) | 94 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected50001.9842.1172.0511.94925001.4021.3261.3641.26212500.7630.8020.7830.6816250.4460.4750.4610.359312.50.2750.2840.2800.178156.20.2010.1930.1970.095780.1640.1570.1610.05900.1060.0980.102 | ||||||
货期：3-5 working days
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