WB | 1/500-1/1000 | Human,Mouse,Rat |
IF | 1/20 | Human,Mouse,Rat |
IHC | 咨询技术 | Human,Mouse,Rat |
ICC | 1/50-1/200 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | MAP3K2; MAPKKK2; MEKK2; Mitogen-activated protein kinase kinase kinase 2; MAPK/ERK kinase kinase 2; MEK kinase 2; MEKK 2 |
Entrez GeneID | 10746 |
WB Predicted band size | Calculated MW: 70 kDa; Observed MW: 70 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Mouse,Rat |
Immunogen | A synthetic peptide of human MEKK2 |
Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3条关于MEKK2抗体的示例参考文献(内容为虚构,仅供格式参考):
1. **文献名称**: "MEKK2 regulates inflammatory signaling via MAP kinase pathways"
**作者**: Smith A, et al.
**摘要**: 本研究利用特异性MEKK2抗体,通过免疫沉淀和免疫印迹技术,揭示MEKK2在TLR4介导的NF-κB和JNK信号通路中的关键作用,证实其在巨噬细胞炎症反应中的调控机制。
2. **文献名称**: "MEKK2 ablation impairs cardiac development in mice"
**作者**: Zhang L, et al.
**摘要**: 通过构建MEKK2基因敲除小鼠模型,结合MEKK2抗体进行组织免疫染色,发现MEKK2缺失导致胚胎期心脏瓣膜发育异常,提示其与Wnt/β-catenin通路的相互作用。
3. **文献名称**: "MEKK2-dependent phosphorylation drives cancer cell invasion"
**作者**: Kim H, et al.
**摘要**: 在乳腺癌细胞中,使用MEKK2抗体阻断实验及shRNA沉默技术,证明MEKK2通过磷酸化下游靶蛋白MMP9.促进肿瘤细胞迁移和侵袭,为潜在治疗靶点提供依据。
注:以上文献为示例,实际引用请查询PubMed等数据库获取真实论文。
The MEKK2 (Mitogen-Activated Protein Kinase Kinase Kinase 2) antibody is a crucial tool for studying the MEKK2 protein, a member of the MAP3K family within the MAPK signaling pathway. MEKK2 plays a pivotal role in regulating cellular responses to stress, cytokines, and growth factors, influencing processes like proliferation, differentiation, and apoptosis. It activates downstream kinases, including MKK5 and MEK1. which subsequently trigger ERK, JNK, or p38 pathways. Dysregulation of MEKK2 is linked to inflammatory diseases, immune disorders, and cancer, making it a target for therapeutic research.
MEKK2 antibodies are typically developed in hosts such as rabbits or mice, targeting specific epitopes within the kinase domain or regulatory regions. These antibodies enable detection of MEKK2 expression and phosphorylation status via techniques like Western blotting, immunoprecipitation, and immunofluorescence. They are also used to study MEKK2’s interaction with signaling partners (e.g., MEKK3) or its role in pathways like NF-κB activation.
Researchers must validate antibody specificity using knockout controls or siRNA knockdown, as cross-reactivity with homologous kinases (e.g., MEKK3) can occur. Applications span basic research—exploring MEKK2’s function in development or disease—and drug discovery, where inhibitors targeting MEKK2 are assessed. Commercial antibodies often provide data on reactivity across human, mouse, and rat samples, ensuring broad experimental utility. Proper storage (often at -20°C) and adherence to dilution protocols are critical for optimal performance.
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