首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人极低密度脂蛋白受体(VLDLR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的VLDLR含量。试剂盒检测范围为1.25 ng/mL-80 ng/mL，可应用于心血管疾病模型研究、脂代谢调控机制探索或药物开发中的靶点效应评估等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FLJ35024 ELISA Kit; Very low density lipoprotein receptor ELISA Kit; Very low-density lipoprotein receptor ELISA Kit; VLDL R ELISA Kit; VLDL receptor ELISA Kit; VLDL-R ELISA Kit; VLDLR ELISA Kit; VLDLR_HUMAN ELISA Kit; VLDLRCH ELISA Kit
缩写：VLDLR
Uniprot No.：P98155
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：1.25 ng/mL-80 ng/mL
灵敏度：0.31 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human VLDLR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of human VLDLR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected802.6122.6992.6562.484402.1732.1072.1401.968201.4281.4381.4331.261100.8980.8420.8700.69850.5460.5690.5580.3862.50.3930.3890.3910.2191.250.2750.2790.2770.10500.1710.1730.172 | ||||||
货期：3-5 working days
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