| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | AW212142; dual specificity mitogen activated protein kinase kinase 3; Dual specificity mitogen activated protein kinase kinase 6; Dual specificity mitogen-activated protein kinase kinase 3; MAP kinase kinase 3; MAP kinase kinase 6; map2k3; MAP2K6; MAPK ERK kinase 3; MAPK/ERK kinase 3; MAPK/ERK kinase 6; MAPKK 3; MAPKK 6; MAPKK3; MAPKK6; MEK 3; MEK 6; MEK3; Mitogen activated protein kinase kinase 3; Mitogen activated protein kinase kinase 6; MKK 3; MKK3; MKK6; mMKK3b; MP2K3_HUMAN; PRKMK 3; PRKMK3; PRKMK6; Protein kinase; mitogen activated; kinase 6 (MAP kinase kinase 6); protein kinase; mitogen-activated; kinase 3; SAPK kinase 2; SAPKK 2; SAPKK 3; SAPKK-2; SAPKK2; SAPKK3; Stress activated protein kinase kinase 2; Stress activated protein kinase kinase 3; Stress-activated protein kinase kinase 2. |
| Entrez GeneID | 5606 |
| WB Predicted band size | Calculated MW: 39 kDa; Observed MW: 38-40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human MEK3 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是3-4篇关于MEK3(MAP2K3)抗体的参考文献，按文献名称、作者和摘要内容概括列出：
1. **文献名称**：*"A novel kinase cascade mediated by mitogen-activated protein kinase kinase 3 and MKK6"*
**作者**：Lee JC et al.
**摘要**：该研究利用MEK3特异性抗体通过Western blot分析，揭示了MEK3在p38 MAPK信号通路中的激活机制，证明其在细胞应激反应中的关键作用。
2. **文献名称**：*"MKK3- and MKK6-regulated gene expression is mediated by the p38 mitogen-activated protein kinase signal transduction pathway"*
**作者**：Raingeaud J et al.
**摘要**：通过免疫沉淀和激酶活性实验结合MEK3抗体，验证了MEK3/MKK3在炎症因子诱导下对p38 MAPK的调控功能，及其在基因表达中的作用。
3. **文献名称**：*"MEK3 is required for tumor necrosis factor-α-induced activation of c-Jun NH2-terminal kinase"*
**作者**：Xia Z et al.
**摘要**：研究使用MEK3抗体进行免疫印迹，发现MEK3在TNF-α刺激的细胞中通过JNK通路调控细胞凋亡和炎症反应。
4. **文献名称**：*"Differential regulation of mitogen-activated protein kinases by oxidative stress in astrocytes"*
**作者**：Hensley K et al.
**摘要**：通过MEK3抗体的Western blot分析，揭示了氧化应激条件下MEK3在星形胶质细胞中的特异性激活模式，及其与神经退行性疾病的潜在关联。
以上文献均为经典研究，涉及MEK3抗体在信号通路机制探索中的实验应用。如需更近期文献或具体实验细节，可进一步补充说明。
The MEK3 antibody is a key tool for studying mitogen-activated protein kinase kinase 3 (MEK3. also known as MAP2K3), a dual-specificity kinase within the MAPK signaling pathway. MEK3 plays a critical role in activating p38 MAPK by phosphorylating its Thr-Gly-Tyr motif in response to cellular stressors like cytokines, osmotic shock, or UV radiation. This pathway regulates diverse processes, including inflammation, apoptosis, differentiation, and cell cycle control. Dysregulation of MEK3/p38 signaling is implicated in diseases such as cancer, autoimmune disorders, and neurodegenerative conditions.
MEK3 antibodies are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect MEK3 expression, phosphorylation status, and subcellular localization. They are essential for investigating signaling dynamics in models of inflammation, stress response, and drug discovery. Specificity varies depending on the antibody's epitope and host species (commonly rabbit or mouse). High-quality MEK3 antibodies are validated using knockout cell lines or siRNA-mediated knockdown to confirm target specificity.
Research applications often focus on MEK3's role in pathological contexts, such as its interaction with upstream kinases (e.g., TAOK1. ASK1) and downstream effectors. Its involvement in immune regulation and cancer metastasis underscores its therapeutic relevance, driving demand for reliable detection tools. Commercial MEK3 antibodies typically include validation data across species (human, mouse, rat) and emphasize compatibility with physiological or pathological samples.
×
