首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠极低密度脂蛋白(VLDL)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的VLDL含量。极低密度脂蛋白（VLDL）是一种运输内源性甘油三酯的脂蛋白。其代谢异常与多种疾病相关，如动脉粥样硬化。研究机制主要聚焦于其合成、分泌、代谢途径，以及和相关受体、酶的相互作用，以探索调控VLDL水平防治疾病的新策略。试剂盒检测范围为3.12 ng/mL-200 ng/mL，广泛应用于肥胖、糖尿病、动脉粥样硬化等代谢性疾病的基础研究，也可用于评估药物干预或基因调控对脂蛋白代谢的影响，为探索相关疾病的分子机制及潜在治疗靶点提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：VLDL
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat VLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 99 | ||||
| Range % | 91-103 | |||||
| 1:200 | Average % | 87 | ||||
| Range % | 81-92 | |||||
| 1:400 | Average % | 104 | ||||
| Range % | 98-107 | |||||
| 1:800 | Average % | 101 | ||||
| Range % | 92-105 | |||||
回收率：
| The recovery of rat VLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 86-99 | ||||
| EDTA plasma (n=4) | 98 | 91-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 2000.0840.0880.086 1000.1520.1590.156 500.2260.2340.230 250.3890.3780.384 12.50.6130.6350.624 6.250.8300.8430.837 3.121.1381.1171.128 01.9701.8691.920 | ||||||
货期：3-5 working days
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