首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠极低密度脂蛋白(VLDL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的VLDL含量。VLDL即极低密度脂蛋白，在人体脂质代谢中发挥重要作用。它由肝脏合成并分泌，能运输内源性甘油三酯至外周组织。研究发现，其代谢异常与动脉粥样硬化等心血管疾病密切相关，深入探究VLDL代谢机制对相关疾病防治意义重大。试剂盒检测范围为0.78 ng/mL-50 ng/mL，适用于代谢机制研究、药物干预效果评估或营养学模型中脂质代谢动态监测，为心血管疾病或代谢综合征基础研究提供高效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：VLDL
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse VLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 92 | ||||
| Range % | 86-98 | |||||
| 1:200 | Average % | 99 | ||||
| Range % | 92-106 | |||||
| 1:400 | Average % | 83 | ||||
| Range % | 80-85 | |||||
| 1:800 | Average % | 89 | ||||
| Range % | 83-97 | |||||
回收率：
| The recovery of mouse VLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-97 | ||||
| EDTA plasma (n=4) | 95 | 91-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected502.1581.9992.0791.994251.3441.2761.3101.22512.50.7420.7040.7230.6386.250.4020.3970.4000.3153.120.2740.2540.2640.1791.560.2010.1960.1990.1140.780.1440.1390.1420.05700.0850.0840.085 | ||||||
货期：3-5 working days
×
