首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠酪氨酸酶(TYR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的TYR含量。TYR即酪氨酸酶，是黑色素合成的关键限速酶。其背景在于它在皮肤、眼睛等部位黑素细胞内发挥重要作用。研究机制上，主要聚焦于其催化酪氨酸转化为多巴及后续反应的过程，通过调控其活性有望为美白、治疗色素相关疾病等提供策略。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，广泛应用于黑色素生成调控机制研究、皮肤疾病动物模型评估、化妆品美白成分筛选等科研领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tyr; Tyrosinase; Albino locus protein; Monophenol monooxygenase
缩写：TYR
Uniprot No.：P11344
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TYR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 93-99 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 84-92 | |||||
| 1:4 | Average % | 101 | ||||
| Range % | 98-104 | |||||
| 1:8 | Average % | 86 | ||||
| Range % | 83-89 | |||||
回收率：
| The recovery of mouse TYR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 87-99 | ||||
| EDTA plasma (n=4) | 98 | 95-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.1562.1692.1632.03810001.5781.5981.5881.4635001.0311.0041.0180.8932500.6420.6350.6390.5141250.4100.4350.4230.29862.50.3160.3260.3210.19631.250.2010.2160.2090.08400.1240.1260.125 | ||||||
货期：3-5 working days
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