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Mouse Thioredoxin-interacting protein(TXNIP) ELISA kit

  • 中文名: 小鼠硫氧还蛋白互作蛋白(TXNIP)酶联免疫试剂盒
货号: IPD-EL025383MO
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    小鼠硫氧还蛋白互作蛋白(TXNIP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的TXNIP含量。TXNIP 是重要靶点。它参与细胞内多种生理过程,如葡萄糖与脂质代谢、氧化应激、细胞凋亡等。当前研究聚焦于其调控机制,探索其在糖尿病、癌症等疾病发生发展中的作用,有望为相关疾病的治疗提供新方向。试剂盒检测范围为0.45 ng/ml-30 ng/ml.,适用于科研领域中对TXNIP表达水平的功能性研究,例如探索氧化应激相关信号通路、代谢调控机制或疾病模型的分子机制验证,为心血管疾病、神经退行性疾病及能量代谢相关研究提供可靠的定量分析工具本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    Txnip ELISA Kit; Vdup1 ELISA Kit; Thioredoxin-interacting protein ELISA Kit; Vitamin D3 up-regulated protein 1 ELISA Kit
  • 缩写:
    TXNIP
  • Uniprot No.:
    Q8BG60
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.45 ng/ml-30 ng/ml.
  • 灵敏度:
    0.11 ng/ml
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cell Biology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse TXNIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    92


    Range %

    85-99


    1:2

    Average %

    95


    Range %

    88-102


    1:4

    Average %

    89


    Range %

    82-96


    1:8

    Average %

    96


    Range %

    90-100


  • 回收率:

    The recovery of mouse TXNIP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    96

    90-101


    EDTA plasma (n=4)

    90

    84-96


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    30

    1.887

    1.794

    1.841

    1.676


    15

    1.427

    1.467

    1.447

    1.282


    7.5

    1.146

    1.078

    1.112

    0.947


    3.75

    0.882

    0.829

    0.855

    0.690


    1.8

    0.522

    0.511

    0.517

    0.352


    0.9

    0.332

    0.343

    0.338

    0.173


    0.45

    0.283

    0.284

    0.284

    0.119


    0

    0.165

    0.165

    0.165




  • 货期:
    3-5 working days

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