首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人胸腺嘧啶核苷磷酸化酶(TP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TYMP含量。TYMP即胸苷磷酸化酶，在血管生成、细胞增殖与凋亡等生理过程中发挥作用。它通过催化胸苷分解影响核苷酸代谢。研究发现其表达异常与多种肿瘤的发生、发展和转移相关，有望成为抗肿瘤治疗的潜在靶点，目前正研究相关药物调控其活性。试剂盒检测范围为62.5 ng/mL-4000 ng/mL，该产品可为肿瘤发生机制研究、血管生成调控实验以及抗肿瘤药物开发等科研领域提供精准的TP蛋白定量数据，尤其适用于探讨肿瘤微环境中TP的表达特征，或通过体液样本（如血清/血浆）评估生物标志物水平变化，为分子生物学及病理学研究提供标准化实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ECGF 1 ELISA Kit; ECGF ELISA Kit; ECGF1 ELISA Kit; Endothelial cell growth factor 1 ELISA Kit; Endothelial cell growth factor 1 platelet derived ELISA Kit; Endothelial cell growth factor, platelet-derived ELISA Kit; Gliostatin ELISA Kit; hPD ECGF ELISA Kit; MEDPS1 ELISA Kit; MNGIE ELISA Kit; MTDPS1 ELISA Kit; PD ECGF ELISA Kit; PD-ECGF ELISA Kit; PDECGF ELISA Kit; PDEGF ELISA Kit; Platelet derived endothelial cell growth factor ELISA Kit; Platelet derived endothelial growth factor ELISA Kit; Platelet-derived endothelial cell growth factor ELISA Kit; TdRPase ELISA Kit; Thymidine phosphorylase ELISA Kit; TP ELISA Kit; Tymp ELISA Kit; TYPH_HUMAN ELISA Kit
缩写：TYMP
Uniprot No.：P19971
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 ng/mL-4000 ng/mL
灵敏度：15.6 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 88-94 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-103 | |||||
| 1:4 | Average % | 101 | ||||
| Range % | 96-106 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 81-95 | |||||
回收率：
| The recovery of human TP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-95 | ||||
| EDTA plasma (n=4) | 102 | 98-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected40002.5212.6942.6082.44520002.1712.0622.1171.95410001.4231.5411.4821.3195000.8550.8580.8570.6942500.6110.6330.6220.4591250.4020.4460.4240.26162.50.2980.2920.2950.13200.1590.1660.163 | ||||||
货期：3-5 working days
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