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产品描述：人硫氧还蛋白互作蛋白(TXNIP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TXNIP含量。TXNIP 即硫氧还蛋白相互作用蛋白，是氧化应激和代谢的关键调节因子。研究表明，它通过与硫氧还蛋白结合抑制其活性来调节氧化还原稳态，还参与糖代谢、炎症反应等过程。在糖尿病、癌症等疾病发生发展机制研究中备受关注 。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，该产品可广泛应用于基础医学研究领域，例如探究糖尿病模型中TXNIP对胰岛β细胞氧化损伤的调控机制，或分析心血管疾病动物组织内TXNIP与炎症因子的相关性，为氧化应激相关疾病的分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TXNIP; VDUP1; Thioredoxin-interacting protein; Thioredoxin-binding protein 2; Vitamin D3 up-regulated protein 1
缩写：TXNIP
Uniprot No.：Q9H3M7
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TXNIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 87-97 | |||||
回收率：
| The recovery of human TXNIP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 94-101 | ||||
| EDTA plasma (n=4) | 86 | 82-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.6612.5342.5982.473 5002.0052.1542.0801.955 2501.2071.2541.2311.106 1250.7200.7410.7310.606 62.50.4050.4160.4110.286 31.20.2830.2880.2860.161 15.60.1890.1840.1870.062 00.1240.1250.125 | ||||||
货期：3-5 working days
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