首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人络氨酸酶(TYR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TYR含量。TYR即酪氨酸酶，它是黑素合成的关键限速酶。在体内能催化酪氨酸转化为黑色素。其研究机制主要聚焦于调控黑素生成过程，包括对其活性、表达水平的影响。通过研究抑制或激活TYR，有助于开发美白、治疗色素异常疾病的药物。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于体外培养模型、动物实验样本或临床前研究中TYR表达水平的定量评估，如黑色素生成调控、皮肤病理模型建立或药物干预效果评价等科研场景，为探索TYR在细胞代谢、皮肤生理及病理过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ATN ELISA Kit; CMM8 ELISA Kit; LB24 AB ELISA Kit; LB24-AB ELISA Kit; Monophenol monooxygenase ELISA Kit; OCA1 ELISA Kit; OCA1A ELISA Kit; OCAIA ELISA Kit; Oculocutaneous albinism IA ELISA Kit; SHEP3 ELISA Kit; SK29 AB ELISA Kit; SK29-AB ELISA Kit; Tumor rejection antigen AB ELISA Kit; TYR ELISA Kit; TYRO_HUMAN ELISA Kit; tyrosinase (oculocutaneous albinism IA) ELISA Kit; Tyrosinase ELISA Kit
缩写：TYR
Uniprot No.：P14679
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TYR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 85 | ||||
| Range % | 81-90 | |||||
| 1:10 | Average % | 89 | ||||
| Range % | 86-92 | |||||
| 1:20 | Average % | 100 | ||||
| Range % | 97-103 | |||||
| 1:40 | Average % | 83 | ||||
| Range % | 80-86 | |||||
回收率：
| The recovery of human TYR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 83-90 | ||||
| EDTA plasma (n=4) | 95 | 91-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.6762.6982.6872.519 5002.0742.1982.1361.968 2501.4371.4561.4471.279 1250.8020.8320.8170.649 62.50.5010.5350.5180.350 31.20.3740.3980.3860.218 15.60.2370.2540.2460.078 00.1670.1690.168 | ||||||
货期：3-5 working days
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