首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠硫氧还蛋白互作蛋白(TXNIP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的TXNIP含量。TXNIP是一种硫氧还蛋白相互作用蛋白。背景上，它在多种细胞生理过程中发挥作用。研究机制方面，其可与硫氧还蛋白相互作用，影响氧化还原平衡。在代谢、炎症等多种病理生理过程中也有参与，是疾病研究中的一个重要潜在靶点。试剂盒检测范围为47 pg/mL-3000 pg/mL，本试剂盒适用于体外实验研究，可广泛用于探索TXNIP在代谢性疾病模型、心血管病理研究或神经退行性疾病中的动态变化，支持从体液到组织/细胞层面的多维度分析。为科研人员研究TXNIP相关信号通路及疾病机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Txnip ELISA Kit; Vdup1 ELISA Kit; Thioredoxin-interacting protein ELISA Kit; Vitamin D3 up-regulated protein 1 ELISA Kit
缩写：TXNIP
Uniprot No.：Q5M7W1
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：47 pg/mL-3000 pg/mL
灵敏度：11.75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TXNIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 91 | |||||
| Range % | 86-95 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 97-107 | ||||||
| 1:4 | Average % | 91 | |||||
| Range % | 85-97 | ||||||
| 1:8 | Average % | 97 | |||||
| Range % | 91-103 | ||||||
回收率：
| The recovery of rat TXNIP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 85-96 | |||||
| EDTA plasma (n=4) | 92 | 87-97 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected30002.5072.5362.5222.34815001.9641.8341.8991.7257501.2781.3041.2911.1173750.7350.7300.7330.559187.50.4820.4920.4870.313940.3640.3720.3680.194470.2670.2610.2640.09000.1730.1750.174 | |||||||
货期：3-5 working days
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