首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠硫氧化还原蛋白(Trx)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TXN含量。试剂盒检测范围为3.12 ng/mL-200 ng/mL，适用于氧化应激相关机制研究的科研场景；适用于研究大鼠疾病模型（如缺血再灌注损伤、神经退行性病变等）中Trx的动态变化，或评估抗氧化药物干预效果，为氧化应激相关分子机制探索提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Txn ELISA Kit; Txn1 ELISA Kit; Thioredoxin ELISA Kit; Trx ELISA Kit
缩写：TXN
Uniprot No.：P11232
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Trx in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:2 | Average % | 91 | ||||
| Range % | 86-97 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of rat Trx spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 102 | 97-109 | ||||
| EDTA plasma (n=4) | 96 | 91-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected2002.4122.3122.3622.2421001.7221.6321.6771.557501.1681.1931.1811.061250.7260.7490.7380.61812.50.4800.4510.4660.3466.250.2860.2750.2810.1613.120.2290.2140.2220.10200.1220.1180.120 | ||||||
货期：3-5 working days
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