首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：牛促甲状腺素(TSH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TSH含量。TSH即促甲状腺激素，是腺垂体分泌的促进甲状腺生长和机能的激素。其可调控甲状腺细胞的增殖与甲状腺血液供应。研究多围绕其与甲状腺疾病如甲亢、甲减的关系展开，明确其作用机制有助于疾病的诊断、治疗及病情监测。试剂盒检测范围为0.9 uIU/mL-60 uIU/mL，适用于牛甲状腺相关疾病机制研究、药物干预效果评估或畜牧养殖中生理状态监测等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TSHB ELISA Kit; Thyrotropin subunit beta ELISA Kit; Thyroid-stimulating hormone subunit beta ELISA Kit; TSH-B ELISA Kit; TSH-beta ELISA Kit; Thyrotropin beta chain ELISA Kit
缩写：TSH
Uniprot No.：P01223
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：0.9 uIU/mL-60 uIU/mL
灵敏度：0.225 μIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine TSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 84-92 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-98 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 88-96 | |||||
回收率：
| The recovery of bovine TSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 95-104 | ||||
| EDTA plasma (n=4) | 84 | 80-88 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| µIU/mlOD1OD2AverageCorrected601.8151.7311.7731.626301.2791.2371.2581.111150.9340.9270.9310.7847.50.6230.6710.6470.5003.750.4080.3790.3940.2471.80.3150.2970.3060.1590.90.2010.1950.1980.05100.1500.1430.147 | ||||||
货期：3-5 working days
×
