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产品描述：大鼠肿瘤坏死因子诱导蛋白6（TSG-6）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TSG-6含量。大鼠肿瘤坏死因子诱导蛋白6（TSG-6）是一种分泌蛋白，具有透明质酸结合域，参与细胞外基质稳定和细胞迁移。它通过增强α-间抑制剂（IαI）的活性，在炎症相关的蛋白酶网络中发挥重要作用。TSG-6的表达受TNF-α和IL-1等信号分子诱导，并与蛋白多糖合成和聚集有关。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于基础科研中探索炎症性疾病机制、评估组织损伤修复模型或肿瘤相关研究中的TSG - 6动态变化本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TSG-6, TSG6, hyaluronate-binding protein|tumor necrosis factor alpha-inducible protein 6|tumor necrosis factor-inducible protein 6|tumor necrosis factor-stimulated gene-6 protein
缩写：TSG-6
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TSG-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 87-95 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 97-104 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 87-94 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 96-104 | |||||
回收率：
| The recovery of rat TSG-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 91-98 | ||||
| EDTA plasma (n=4) | 94 | 91-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.5452.5322.5392.373 101.9031.8671.8851.719 51.2101.2221.2161.050 2.50.6830.7180.7010.535 1.250.4290.4480.4390.273 0.6250.3410.3320.3370.171 0.3120.2560.2430.2500.084 00.1680.1640.166 | ||||||
货期：3-5 working days
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