| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HSP72; HSPA1; HSP70I; HSPA1B; HSP70-1; HSP70-1A. |
| Entrez GeneID | 3303 |
| WB Predicted band size | Calculated MW: 70 kDa; Observed MW: 70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human Hsp70 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3条关于Hsp70抗体的参考文献及其摘要概括：
1. **文献名称**：*Hsp70 chaperones: Cellular functions and molecular mechanisms*
**作者**：Hartl, F.U., & Hayer-Hartl, M.
**摘要**：该综述总结了Hsp70家族蛋白在细胞应激反应中的关键作用，包括协助蛋白质折叠、防止聚集及促进错误折叠蛋白降解。文中强调了Hsp70抗体在检测其亚细胞定位和表达水平中的应用，为研究其分子机制提供了实验依据。
2. **文献名称**：*Heat shock protein 70 (Hsp70) as a diagnostic marker in cancer*
**作者**：Ciocca, D.R., & Calderwood, S.K.
**摘要**：研究探讨了Hsp70在多种肿瘤中的过表达现象，提出其作为癌症生物标志物的潜力。作者通过免疫组化和Western blot(使用特异性Hsp70抗体)验证了其在乳腺癌、肺癌等组织样本中的高表达，并关联其与患者预后的关系。
3. **文献名称**：*Selective targeting of inducible HSP70 by a antibody-based probe in stressed cells*
**作者**：Murphy, M.E., et al.
**摘要**：该研究开发了一种高特异性的Hsp70单克隆抗体，用于区分基础状态与应激诱导的Hsp70表达。实验证明该抗体在流式细胞术和免疫荧光中具有高灵敏性，为动态监测细胞应激反应提供了新工具。
(注：以上文献信息为示例，实际引用需核实具体来源及内容。)
Hsp70 antibodies are essential tools for studying the heat shock protein 70 (Hsp70) family, a group of evolutionarily conserved molecular chaperones critical for cellular stress responses and protein homeostasis. Hsp70 proteins, including isoforms like HSPA8 (cytosolic), GRP78/BiP (endoplasmic reticulum), and HSPA9/mtHsp70 (mitochondrial), assist in protein folding, prevent aggregation of misfolded proteins, and promote cell survival under stress conditions such as heat, oxidative stress, or hypoxia. Their structure comprises an N-terminal ATPase domain and a C-terminal substrate-binding domain, enabling ATP-dependent binding to exposed hydrophobic regions of client proteins.
Hsp70 antibodies are widely used in techniques like Western blot, immunohistochemistry, and immunofluorescence to detect Hsp70 expression, localization, and interactions. These antibodies help investigate roles of Hsp70 in diseases, including cancer (where Hsp70 is often overexpressed to support tumor survival), neurodegenerative disorders (e.g., Alzheimer’s and Parkinson’s diseases linked to protein misfolding), and infections (pathogens exploit host Hsp70 for replication). Researchers also employ them to study Hsp70’s dual role in apoptosis—either promoting cell death or survival, depending on context.
Due to high sequence homology among Hsp70 isoforms, antibody specificity is critical. Many commercial antibodies target unique regions of specific isoforms or stress-inducible forms like HSPA1A/B. Validation using knockout cells or recombinant proteins ensures reliability. Beyond research, Hsp70 antibodies aid in developing therapeutic strategies, such as Hsp70 inhibitors for cancer or Hsp70-based vaccines. Their utility in diagnostics and drug development underscores their importance in biomedical research.
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