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产品描述：人肿瘤易感基因101蛋白(TSG101)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的TSG101含量。TSG101是一个重要靶点。它在细胞内参与多种生理过程，如囊泡运输等。研究发现其异常表达与肿瘤等疾病的发生发展密切相关。目前针对TSG101的研究主要聚焦于其作用机制及作为治疗靶点开发新的治疗策略。试剂盒检测范围为23.44 pg/mL-1500 pg/mL，本产品适用于基础科研领域，如肿瘤微环境研究、病毒出芽机制探索、细胞囊泡运输相关实验，以及通过检测不同处理条件下细胞或组织中TSG101的动态变化，为分子调控机制研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ESCRT I complex subunit TSG101 ELISA Kit; ESCRT-I complex subunit TSG101 ELISA Kit; TS101_HUMAN ELISA Kit; TSG 10 ELISA Kit; TSG 101 ELISA Kit; TSG10 ELISA Kit; Tsg101 ELISA Kit; Tumor susceptibility 101 ELISA Kit; Tumor susceptibility gene 10 ELISA Kit; Tumor susceptibility gene 101 ELISA Kit; Tumor susceptibility gene 101 protein ELISA Kit; Tumor susceptibility protein ELISA Kit; Tumor susceptibility protein isoform 3 ELISA Kit; VPS 23 ELISA Kit; VPS23 ELISA Kit
缩写：TSG101
Uniprot No.：Q99816
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.44 pg/mL-1500 pg/mL
灵敏度：5.86 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TSG101 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 98 | |||||
| Range % | 92-104 | ||||||
| 1:2 | Average % | 108 | |||||
| Range % | 103-112 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 90-98 | ||||||
| 1:8 | Average % | 89 | |||||
| Range % | 86-95 | ||||||
回收率：
| The recovery of human TSG101 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 102 | 99-106 | |||||
| EDTA plasma (n=4) | 94 | 90-98 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected15001.8721.9211.8971.7877501.3221.3881.3551.2453750.9540.9020.9280.818187.50.6080.6220.6150.50593.750.4170.4120.4150.30546.880.2890.2870.2880.17823.440.2300.2440.2370.12700.1090.1110.110 . | |||||||
货期：3-5 working days
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