| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | High temperature requirement protein A2; HTRA 2; HtrA like serine protease; HtrA serine peptidase 2; HtrA; E. coli; homolog of; 2; HtrA2; HTRA2_HUMAN; mitochondrial; Omi stress regulated endoprotease; Omi stress-regulated endoprotease; PARK 13; PARK13; Protease serine 25; PRSS 25; PRSS25; Serine protease 25; Serine protease HTRA2; Serine protease HTRA2 mitochondrial; Serine protease htra2 mitochondrial precursor; Serine protease omi; Serine proteinase OMI. |
| Entrez GeneID | 27429 |
| WB Predicted band size | Calculated MW: 49 kDa; Observed MW: 36 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthetic peptide of human HtrA2/Omi |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于HtrA2抗体的3篇文献及其摘要内容的简要整理：
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1. **文献名称**：*HtrA2 promotes cell death through its serine protease activity and its ability to antagonize inhibitor of apoptosis proteins*
**作者**：Suzuki Y, et al.
**期刊/年份**：*Molecular Cell*, 2001.
**摘要内容**：
该研究揭示了HtrA2/Omi蛋白酶通过切割凋亡抑制蛋白(IAPs)促进细胞凋亡的机制。作者利用特异性抗体证实HtrA2在应激条件下从线粒体释放至胞质，并通过其丝氨酸蛋白酶活性直接参与caspase依赖和非依赖的凋亡通路。
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2. **文献名称**：*Structural and functional basis of the serine protease HtrA2/Omi quality control system*
**作者**：Clausen T, et al.
**期刊/年份**：*Nature Structural & Molecular Biology*, 2011.
**摘要内容**：
研究通过X射线晶体学解析了HtrA2的三维结构，并利用抗体进行功能验证，表明其通过PDZ结构域识别错误折叠蛋白，进而激活蛋白酶活性。该研究为HtrA2在神经退行性疾病(如帕金森病)中的作用提供了分子机制解释。
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3. **文献名称**：*Loss of HtrA2/Omi activity in Parkinson’s disease and schizophrenia*
**作者**：Strauss KM, et al.
**期刊/年份**：*Brain Research*, 2005.
**摘要内容**：
通过免疫组化和Western blot分析，发现帕金森病患者脑组织中HtrA2表达及活性显著降低，提示其功能缺失可能导致线粒体功能障碍和神经元凋亡。研究强调了HtrA2抗体在疾病病理研究中的应用价值。
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**备注**：以上文献可通过PubMed或Google Scholar搜索标题获取全文。若需更多文献或具体应用场景(如抗体品牌/实验方法)，可进一步补充信息。
The HtrA2 antibody is a crucial tool for studying the HtrA2 protein (High-temperature requirement A2), also known as Omi, a serine protease implicated in apoptosis and cellular stress responses. HtrA2 resides predominantly in the mitochondrial intermembrane space, where it plays dual roles: maintaining mitochondrial homeostasis under normal conditions and promoting apoptosis upon cellular stress. During stress, HtrA2 is released into the cytosol, where it cleaves inhibitor of apoptosis proteins (IAPs), thereby activating caspase-dependent apoptosis. Dysregulation of HtrA2 is linked to neurodegenerative disorders, particularly Parkinson’s disease, as mutations (e.g., S399C) impair its protease activity, leading to mitochondrial dysfunction and neuronal death. Additionally, HtrA2’s interaction with PDZ domains and substrates like amyloid-beta highlights its relevance in Alzheimer’s disease and cancer.
HtrA2 antibodies are widely used to detect protein expression, localization, and post-translational modifications via techniques like Western blot, immunohistochemistry, and immunofluorescence. Specific clones (e.g., clone 3F6) target distinct epitopes, enabling diverse research applications. These antibodies aid in exploring HtrA2’s role in disease mechanisms, drug targeting, and biomarker discovery. Validation of antibody specificity, often through knockout controls, is critical due to potential cross-reactivity with homologous proteases (e.g., HtrA1 or HtrA3). Overall, HtrA2 antibodies are indispensable for unraveling the protein’s pathophysiological significance and therapeutic potential.
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