首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人胸苷酸合成酶(TS)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的TYMS含量。TYMS（胸苷酸合成酶）是一个重要的药物靶点。它在DNA合成与修复中扮演关键角色，负责催化脱氧尿苷酸转变为脱氧胸苷酸。对TYMS的研究机制主要聚焦于开发能抑制其活性的药物，以阻碍肿瘤细胞DNA合成，进而达到抗癌目的。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于肿瘤生物学、分子药理学等科研领域，如探究化疗药物（如5 - 氟尿嘧啶）对TS活性的抑制作用、评估肿瘤细胞耐药机制或筛选TS抑制剂等研究。可满足体外实验、动物模型及临床前研究中对TS表达动态监测的需求，为癌症治疗机制探索和药物开发提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：d TMP synthase ELISA Kit; EC 2.1.1.45 ELISA Kit; HsT422 ELISA Kit; MGC88736 ELISA Kit; OTTHUMP00000162195 ELISA Kit; Thymidylate synthase ELISA Kit; Thymidylate synthetase ELISA Kit; TMS ELISA Kit; TS ELISA Kit; TSase ELISA Kit; Tyms ELISA Kit; TYMS protein ELISA Kit; Tyms thymidylate synthetase ELISA Kit; TYSY_HUMAN ELISA Kit
缩写：TS
Uniprot No.：P04818
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 84-91 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 99-105 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 90-98 | |||||
回收率：
| The recovery of human TS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 102 | 98-106 | ||||
| EDTA plasma (n=4) | 87 | 84-90 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.6982.5872.6432.467102.1742.1982.1862.01051.5951.5341.5651.3892.50.8660.8680.8670.6911.250.5510.5680.5600.3840.6250.3650.3810.3730.1970.3120.2810.2740.2780.10200.1750.1770.176 | ||||||
货期：3-5 working days
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