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产品描述：猪胰蛋白酶(Trypsin)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的Trypsin含量。Trypsin即胰蛋白酶，是一种丝氨酸蛋白酶，广泛存在于脊椎动物消化系统中。其能特异性切割精氨酸和赖氨酸残基羧基端肽键。在研究机制上，常作为工具酶用于蛋白质分析，通过切割蛋白质来确定其结构和功能 。试剂盒检测范围为3.12 ng/mL-200 ng/mL，可用于动物模型研究、胰腺外分泌功能评估、饲料营养代谢分析或酶活性相关机制探索等科研场景，为消化生理学、病理学及生物医药开发提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：Trypsin
种属：Sus scrofa (Pig)
样本类型：serum, plasma
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||||||||
| Sample | Serum(n=4) | ||||||||||||
| 1:5 | Average % | 89 | |||||||||||
| Range % | 83-97 | ||||||||||||
| 1:10 | Average % | 85 | |||||||||||
| Range % | 80-90 | ||||||||||||
| 1:20 | Average % | 90 | |||||||||||
| Range % | 84-99 | ||||||||||||
| 1:40 | Average % | 93 | |||||||||||
| Range % | 88-100 | ||||||||||||
回收率：
| The recovery of pig trypsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||||||||
| Sample Type | Average % Recovery | Range | |||||||||||
| Serum (n=5) | 98 | 93-103 | |||||||||||
| EDTA plasma (n=4) | 99 | 94-105 | |||||||||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||
| ng/mlOD1OD2AverageCorrected2002.1922.1312.1622.0291001.7691.8341.8021.669501.2891.3191.3041.171250.8240.8670.8460.71312.50.5430.5770.5600.4276.250.3720.3370.3550.2223.120.2880.2590.2740.14100.1370.1290.133 | |||||||||||||
货期：3-5 working days
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