| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HSF1; HSTF1; Heat shock factor protein 1; HSF 1; Heat shock transcription factor 1; HSTF 1 |
| Entrez GeneID | 3297 |
| WB Predicted band size | Calculated MW: 57 kDa; Observed MW: 80 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human HSF1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于HSF1抗体的3篇参考文献概览：
1. **文献名称**：*HSF1 as a biomarker for thermal stress response in mammalian cells*
**作者**：Smith J, et al.
**摘要**：该研究开发了一种高特异性HSF1单克隆抗体，用于检测热应激条件下哺乳动物细胞中HSF1的核转位和磷酸化状态，验证了其在Western blot和免疫荧光中的应用。
2. **文献名称**：*Antibody-based modulation of HSF1 activity in neurodegenerative disease models*
**作者**：Lee S, et al.
**摘要**：研究利用HSF1抗体通过ChIP-seq技术分析阿尔茨海默病模型中HSF1与DNA的结合活性，发现其调控热休克蛋白HSP70的表达变化，提示潜在治疗靶点。
3. **文献名称**：*Validation of cross-species HSF1 antibodies for comparative stress response studies*
**作者**：Garcia R, et al.
**摘要**：评估了多种市售HSF1抗体在小鼠、果蝇和人类细胞中的交叉反应性，确定了两种适用于多物种研究的抗体，并提供了优化实验条件。
注：以上文献为示例，实际引用需核对真实数据库(如PubMed)并补充DOI等信息。
**Background of HSF1 Antibody**
HSF1 (Heat Shock Factor 1) is a critical transcription factor that regulates the cellular stress response by activating the expression of heat shock proteins (HSPs) under conditions of proteotoxic stress, such as heat shock, oxidative stress, or chemical toxicity. It functions as a master regulator of the heat shock response (HSR), binding to heat shock elements (HSEs) in the promoters of target genes to ensure protein homeostasis (proteostasis).
HSF1 antibodies are essential tools for studying its expression, localization, and activity in various biological contexts. Researchers use these antibodies in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to investigate HSF1’s role in stress adaptation, cancer, neurodegenerative diseases, and aging. In cancer, HSF1 is often hyperactivated, promoting tumor survival, metastasis, and drug resistance by upregulating oncogenic HSPs and stress-response pathways. Conversely, in neurodegenerative disorders like Alzheimer’s or Parkinson’s disease, HSF1 dysfunction is linked to impaired proteostasis and protein aggregation.
HSF1-specific antibodies also aid in exploring post-translational modifications (e.g., phosphorylation) that regulate its transcriptional activity. Their application extends to preclinical studies targeting HSF1 for therapeutic intervention, highlighting their significance in both basic research and translational medicine.
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