| WB | 1/500-1/1000 | Human,Mouse,Rat,Hamster |
| IF | 1/20 | Human,Mouse,Rat,Hamster |
| IHC | 1/50-1/100 | Human,Mouse,Rat,Hamster |
| ICC | 技术咨询 | Human,Mouse,Rat,Hamster |
| FCM | 咨询技术 | Human,Mouse,Rat,Hamster |
| Elisa | 咨询技术 | Human,Mouse,Rat,Hamster |
| Aliases | Heat shock cognate 71 kDa protein (Heat shock 70 kDa protein 8) |
| Entrez GeneID | 3312 |
| WB Predicted band size | Calculated MW: 71 kDa; Observed MW: 71 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Hamster |
| Immunogen | A synthetic peptide of human Hsc70 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Hsc70抗体的3篇代表性文献，按研究主题分类简要概括：
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1. **文献名称**：*Hsc70 chaperone activity is required for the cytosolic slow axonal transport of soluble proteins*
**作者**：B. N. Rao et al.
**摘要**：该研究利用Hsc70特异性抗体结合免疫荧光技术，揭示了Hsc70通过调控分子伴侣活性，促进神经元轴突中可溶性蛋白的慢速运输，并证明其与微管网络的功能关联。
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2. **文献名称**：*Differential expression of Hsc70 and Hsp70 in human tumors and their role in cancer cell survival*
**作者**：M. A. Brodsky et al.
**摘要**：通过Western blot和免疫组化分析多种癌症样本，发现Hsc70(组成型表达)与Hsp70(应激诱导型)在肿瘤中的差异表达，并证实Hsc70抗体可用于评估肿瘤微环境中蛋白质稳态失衡与化疗耐药性的关系。
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3. **文献名称**：*Development and validation of a monoclonal antibody specific for the C-terminal domain of Hsc70*
**作者**：K. Terada et al.
**摘要**：研究团队开发了一种针对Hsc70 C端结构域的单克隆抗体，验证其特异性(不与Hsp70交叉反应)，并应用于免疫共沉淀(Co-IP)实验，解析Hsc70与伴侣蛋白复合物的相互作用机制。
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**领域覆盖**：
- **基础机制**：Hsc70在细胞运输中的作用(文献1)
- **疾病关联**：癌症中Hsc70的功能(文献2)
- **实验工具**：特异性抗体的开发与应用(文献3)
如需扩展或调整方向(如神经退行性疾病、病毒复制机制等)，可进一步补充。
Hsc70 (heat shock cognate 70 kDa protein) is a constitutively expressed member of the HSP70 (heat shock protein 70) family, which plays critical roles in protein homeostasis, folding, and trafficking under both normal and stress conditions. Unlike its stress-inducible counterpart Hsp70. Hsc70 is abundant in most cells under basal conditions, functioning as a molecular chaperone to assist in ATP-dependent folding of nascent polypeptides, refolding of misfolded proteins, and targeted degradation via pathways like chaperone-mediated autophagy. It localizes to the cytoplasm, nucleus, and organelles such as the endoplasmic reticulum, participating in diverse processes including synaptic vesicle recycling, antigen presentation, and endocytosis.
Hsc70 antibodies are widely used in research to study its expression, subcellular distribution, and interactions. These antibodies typically target conserved regions, such as the N-terminal ATPase domain or the C-terminal substrate-binding domain, with specificity verified against recombinant proteins or knockout models. Commercial Hsc70 antibodies (polyclonal or monoclonal) enable applications like Western blotting, immunohistochemistry, and immunoprecipitation. Research has linked Hsc70 dysregulation to neurodegenerative diseases (e.g., Alzheimer’s, Parkinson’s) due to its role in clearing aggregates like tau and α-synuclein. It also exhibits dual roles in cancer, acting as a tumor suppressor by promoting protein quality control or supporting oncogenesis through stress adaptation. Additionally, Hsc70 is exploited by pathogens (e.g., viruses, bacteria) for host cell entry, making it a potential therapeutic target. Its ubiquitous expression and multifunctionality underscore its biological importance and the utility of Hsc70 antibodies in mechanistic studies.
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