首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠胰蛋白酶(trypsin)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的trypsin含量。胰蛋白酶是一种丝氨酸蛋白酶，主要在消化系统中将蛋白质分解为肽和氨基酸。它通过特异性切割赖氨酸和精氨酸残基的肽键来发挥作用，是蛋白质消化和蛋白质组学研究中不可或缺的工具酶。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于科研领域中对胰蛋白酶表达水平的定量分析，例如研究胰腺疾病模型中的酶活性变化、评估细胞培养体系中胰蛋白酶的释放规律，或分析组织样本在特定生理病理条件下的蛋白酶动态。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：trypsin
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 89-95 | |||||
| 1:2 | Average % | 103 | ||||
| Range % | 99-107 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 94-101 | |||||
回收率：
| The recovery of mouse trypsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-96 | ||||
| EDTA plasma (n=4) | 95 | 89-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.2192.1122.1662.010501.6241.5761.6001.444251.0010.9910.9960.84012.50.6790.6660.6730.5176.250.3770.3560.3670.2113.120.3040.3130.3090.1531.560.2600.2730.2670.11100.1550.1570.156 | ||||||
货期：3-5 working days
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