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产品描述:人瞬态电压感受器阳离子通道,子类V,成员4 (TrpV4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆、细胞裂解物样本中的TRPV4含量。TRPV4是一种重要的离子通道蛋白靶点。它广泛分布于人体多种组织器官,参与多种生理病理过程。研究机制方面,其可被多种刺激激活,通过调控细胞内钙离子浓度,影响细胞功能和信号传导,在痛觉、体温调节、渗透压感受等领域是研究热点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL,支持温度刺激、机械应力等细胞模型的功能研究,也可用于疾病动物模型中通道蛋白表达水平动态监测,为神经生物学、骨代谢研究及靶向药物开发提供可靠工具,适用于生命科学基础研究与转化医学探索。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:BCYM3 ELISA Kit; CMT2C ELISA Kit; HMSN2C ELISA Kit; osm 9 like TRP channel 4 ELISA Kit; Osm-9-like TRP channel 4 ELISA Kit; OSM9 like transient receptor potential channel 4 ELISA Kit; Osmosensitive transient receptor potential channel 4 ELISA Kit; OTRPC 4 ELISA Kit; OTRPC4 ELISA Kit; SMAL ELISA Kit; SPSMA ELISA Kit; SSQTL1 ELISA Kit; Transient receptor potential cation channel subfamily V member 4 ELISA Kit; Transient receptor potential protein 12 ELISA Kit; TRP 12 ELISA Kit; TRP12 ELISA Kit; TRPV 4 ELISA Kit; TrpV4 ELISA Kit; TRPV4_HUMAN ELISA Kit; Vanilloid receptor like channel 2 ELISA Kit; Vanilloid receptor like protein 2 ELISA Kit; Vanilloid receptor related osmotically activated channel ELISA Kit; Vanilloid receptor-like channel 2 ELISA Kit; Vanilloid receptor-like protein 2 ELISA Kit; Vanilloid receptor-related osmotically-activated channel ELISA Kit; VR 4 ELISA Kit; VR OAC ELISA Kit; VR-OAC ELISA Kit; VR4 ELISA Kit; VRL 2 ELISA Kit; VRL-2 ELISA Kit; VRL2 ELISA Kit; VROAC ELISA Kit
缩写:TRPV4
Uniprot No.:Q9HBA0
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates, cell lysates
检测范围:15.6 pg/mL-1000 pg/mL
灵敏度:3.9 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Neuroscience
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human TrpV4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 101 | |||||
Range % | 95-108 | ||||||
1:2 | Average % | 85 | |||||
Range % | 80-93 | ||||||
1:4 | Average % | 92 | |||||
Range % | 88-97 | ||||||
1:8 | Average % | 90 | |||||
Range % | 85-94 |
回收率:
The recovery of human TrpV4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 98 | 94-103 | |||||
EDTA plasma (n=4) | 94 | 90-100 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/mlOD1OD2AverageCorrected10002.0041.9251.9651.8405001.6821.6011.6421.5172501.3501.4101.3801.2551251.0231.0521.0380.91362.50.6710.7030.6870.56231.250.4870.4560.4720.34715.60.2720.2640.2680.14300.1280.1210.125 |
货期:3-5 working days
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