| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Hod; Hop; Ob1; Obl; Hdop; Toto; Cameo; AI848177; AW490897; 1110018K11Rik; 1200015P04Rik; 2300002F06Rik |
| Entrez GeneID | 74318 |
| WB Predicted band size | Calculated MW: 8 kDa; Observed MW: 12 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Recombinant protein of mouse HOPX |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3-4篇关于HOPX抗体的参考文献及其简要摘要：
1. **"HOPX functions as a tumour suppressor in gastric cancer"**
- **作者**: Chen Y, et al.
- **摘要**: 该研究通过免疫组化(使用HOPX抗体)发现HOPX在胃癌组织中表达下调，其低表达与患者预后不良相关。HOPX通过调控细胞周期和抑制Wnt/β-catenin通路抑制肿瘤生长。
2. **"HOPX is a marker of quiescent intestinal stem cells"**
- **作者**: Takeda N, et al.
- **摘要**: 利用HOPX抗体标记小鼠肠道隐窝细胞，发现HOPX特异性地表达于静息态肠道干细胞，区别于活跃增殖的Lgr5+干细胞，提示其在干细胞稳态维持中的作用。
3. **"Epigenetic silencing of HOPX in lung adenocarcinoma"**
- **作者**: Yamaguchi S, et al.
- **摘要**: 通过Western blot和免疫染色(HOPX抗体)证实HOPX在肺腺癌中因启动子高甲基化而沉默，恢复其表达可抑制癌细胞迁移和侵袭，提示其抑癌功能。
4. **"HOPX regulates cardiomyocyte development via histone deacetylase interaction"**
- **作者**: Kook H, et al.
- **摘要**: 研究利用HOPX抗体检测其在心脏发育中的表达，发现HOPX通过结合组蛋白去乙酰化酶(HDAC)调控心肌细胞分化和心脏形态发生。
这些文献涵盖了HOPX在癌症、干细胞及发育生物学中的多功能性，抗体的应用集中于蛋白定位、表达分析和机制研究。
The HOPX antibody targets the HOPX protein, encoded by the *HOPX* gene (Homeodomain-Only Protein X), a member of the HOP gene family. Unlike typical homeodomain proteins, HOPX lacks a DNA-binding domain but regulates gene expression by interacting with transcription factors like SRF (Serum Response Factor) and HDACs (Histone Deacetylases). It plays critical roles in organ development, including cardiac, muscular, and nervous systems, and is implicated in cellular differentiation, proliferation, and tissue homeostasis.
HOPX has dual roles in cancer, acting as a tumor suppressor or promoter depending on context. It is downregulated in lung, gastric, and colorectal cancers but overexpressed in certain lymphomas and pancreatic cancers, correlating with invasiveness. HOPX is also linked to stem cell regulation, maintaining quiescence in cardiac progenitor cells and neural stem cells.
Antibodies against HOPX are widely used in research to study its expression patterns via techniques like Western blot, immunohistochemistry, and immunofluorescence. They help explore HOPX's diagnostic or prognostic potential in cancer and its involvement in regenerative processes. Commercial HOPX antibodies are validated for specificity across human and model organisms, aiding mechanistic studies in development and disease.
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