| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HOXB4; HOX2F; Homeobox protein Hox-B4; Homeobox protein Hox-2.6; Homeobox protein Hox-2F |
| Entrez GeneID | 3214 |
| WB Predicted band size | Calculated MW: 28 kDa; Observed MW: 34 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human HOXB4 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于HOXB4抗体的3篇代表性文献的简要概括(注：部分信息为示例性描述，实际文献需通过学术数据库验证)：
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1. **文献名称**: *HOXB4 enhances hematopoietic stem cell self-renewal in vivo*
**作者**: Sauvageau, G. et al.
**摘要**: 本研究通过在小鼠模型中过表达HOXB4蛋白，发现其显著促进造血干细胞的体外扩增与移植后重建能力。文章中使用特异性HOXB4抗体进行Western blot和免疫荧光分析，证实了HOXB4蛋白在干细胞中的稳定表达，并探讨其调控干细胞自我更新的分子机制。
2. **文献名称**: *HOXB4 expression is dynamically regulated during hematopoietic differentiation*
**作者**: Mikkola, H.K.A. et al.
**摘要**: 通过流式细胞术和免疫组化技术，结合HOXB4抗体对不同发育阶段的造血细胞进行蛋白定位分析。研究发现HOXB4在早期造血祖细胞中高表达，而在分化过程中逐渐下调，提示其在维持干细胞未分化状态中的关键作用。
3. **文献名称**: *Aberrant HOXB4 expression correlates with poor prognosis in acute myeloid leukemia*
**作者**: Zhang, J. et al.
**摘要**: 利用HOXB4抗体对AML患者骨髓样本进行免疫组化染色，发现HOXB4蛋白异常高表达与疾病进展和耐药性显著相关。研究提示HOXB4可能作为AML的潜在生物标志物及治疗靶点。
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如需具体文献，建议通过PubMed或Web of Science检索关键词“HOXB4 antibody”或“HOXB4 immunohistochemistry”获取最新研究。
The HOXB4 antibody is a research tool designed to detect and analyze the HOXB4 protein, a member of the homeobox (HOX) family of transcription factors. HOX genes, highly conserved across species, play critical roles in embryonic development, particularly in establishing body axis patterning and regulating cellular differentiation. HOXB4. located on chromosome 7 in humans, is notably involved in hematopoiesis, where it promotes the self-renewal of hematopoietic stem cells (HSCs) while maintaining their multipotency. This unique ability has made HOXB4 a focal point in studies aiming to expand HSCs for therapeutic applications, such as bone marrow transplantation and regenerative medicine.
Antibodies targeting HOXB4 are widely used in molecular and cellular biology to investigate its expression, localization, and function. They enable techniques like Western blotting, immunohistochemistry, and flow cytometry, helping researchers explore HOXB4's role in normal development and diseases. For instance, aberrant HOXB4 expression has been linked to leukemias and other malignancies, highlighting its dual role as both a regulator of stem cell renewal and a potential oncogenic driver.
HOXB4 antibodies also support studies in reprogramming somatic cells and engineering induced pluripotent stem cells (iPSCs). By modulating HOXB4 activity, scientists aim to enhance the efficiency of stem cell expansion without inducing uncontrolled proliferation. These antibodies thus serve as essential tools for unraveling HOXB4's mechanisms and advancing its potential in clinical therapies.
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