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产品描述：犬胰蛋白酶(trypsin)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的trypsin含量。Trypsin即胰蛋白酶，是一种丝氨酸蛋白酶。它在人体消化过程中起关键作用，能水解蛋白质。在研究方面，胰蛋白酶可作为药物靶点，用于开发治疗胰腺炎等疾病的药物，同时也用于一些生物工艺，如细胞培养实验中解离细胞。试剂盒检测范围为39 ng/mL-2500 ng/mL，该产品适用于科研领域中对犬类胰腺生理机制、炎症反应或营养代谢相关课题的探索，例如通过检测不同实验条件下胰蛋白酶表达动态，解析其与疾病模型的关联性或评估药物干预效果。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：trypsin
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：39 ng/mL-2500 ng/mL
灵敏度：9.75 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of canine trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 85-106 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 85-105 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 82-102 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 84-110 | |||||
回收率：
| The recovery of canine trypsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 87-107 | ||||
| EDTA plasma (n=4) | 97 | 86-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected25002.2632.3192.2912.16212501.8611.9541.9081.7796251.3651.3721.3691.2403120.8740.8980.8860.7571560.5920.5830.5880.459780.3070.2980.3030.174390.2320.2460.2390.11000.1270.1310.129 | ||||||
货期：3-5 working days
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