首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人组织因子途径抑制物-2(TFPI-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TFPI2含量。TFPI2即组织因子途径抑制物 2，它是一种丝氨酸蛋白酶抑制剂。其它在多种组织中表达，与肿瘤等疾病发生发展相关。研究机制上，主要是通过抑制相关蛋白酶活性，在细胞增殖、迁移、侵袭等过程发挥调控作用，为疾病治疗提供潜在靶点。试剂盒检测范围为0.78 ng/mL-50 ng/mL，适用于生命科学研究中TFPI - 2的定量分析，如探究其在肿瘤微环境中的作用机制、心血管疾病模型中的表达变化或相关信号通路调控实验本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Placental protein 5 ELISA Kit; PP5 ELISA Kit; REF1 ELISA Kit; Retinal pigment epithelium cell factor 1 ELISA Kit; TFPI 2 ELISA Kit; TFPI-2 ELISA Kit; TFPI2 ELISA Kit; TFPI2 PEN ELISA Kit; TFPI2_HUMAN ELISA Kit; Tissue factor pathway inhibitor 2 ELISA Kit
缩写：TFPI2
Uniprot No.：P48307
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TFPI-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 93-104 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-99 | |||||
| 1:4 | Average % | 84 | ||||
| Range % | 80-89 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 85-95 | |||||
回收率：
| The recovery of human TFPI-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-99 | ||||
| EDTA plasma (n=4) | 89 | 83-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected502.8312.6422.7372.588252.0541.9161.9851.83612.51.3031.3521.3281.1796.250.8070.7570.7820.6333.120.5210.5040.5130.3641.560.3190.3520.3360.1870.780.2170.2010.2090.06000.1450.1530.149 | ||||||
货期：3-5 working days
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