首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠转铁蛋白(TF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TF含量。TF即组织因子，是一种跨膜糖蛋白。它是凝血系统的重要启动因子，在血管损伤时启动外源性凝血途径。研究机制主要围绕其在凝血级联反应中的作用展开，同时在炎症、肿瘤转移等病理过程中也有重要作用，是潜在的治疗靶点。试剂盒检测范围为7.8 pmol/mL-500 pmol/mL，广泛应用于铁代谢研究、肝脏功能评估、营养干预实验以及疾病动物模型（如贫血、慢性炎症或铁过载相关病理）的机制探索，为体外实验提供可靠的生物标志物检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tf ELISA Kit; Serotransferrin ELISA Kit; Transferrin ELISA Kit; Beta-1 metal-binding globulin ELISA Kit; Liver regeneration-related protein LRRG03 ELISA Kit; Siderophilin ELISA Kit
缩写：TF
Uniprot No.：P12346
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 pmol/mL-500 pmol/mL
灵敏度：1.95 pmol/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 89 | ||||
| Range % | 85-92 | |||||
| 1:200 | Average % | 96 | ||||
| Range % | 89-104 | |||||
| 1:400 | Average % | 90 | ||||
| Range % | 84-96 | |||||
| 1:800 | Average % | 93 | ||||
| Range % | 87-99 | |||||
回收率：
| The recovery of rat TF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-98 | ||||
| EDTA plasma (n=4) | 94 | 89-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pmol/mlOD1OD2AverageCorrected5002.0422.1432.0931.9582501.6821.6961.6891.5541251.1971.2341.2161.08162.50.8030.8190.8110.67631.20.3920.4070.4000.26515.60.2600.2980.2790.1447.80.1730.1800.1770.04200.1340.1360.135 | ||||||
货期：3-5 working days
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