| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Aspartate aminotransferase; cytoplasmic; Glutamate oxaloacetate transaminase 1; Transaminase A |
| Entrez GeneID | 2805 |
| WB Predicted band size | Calculated MW: 46 kDa; Observed MW: 46 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human Aspartate Aminotransferase |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于天冬氨酸氨基转移酶(AST)抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*"Production and characterization of monoclonal antibodies against human aspartate aminotransferase"*
**作者**：Smith J, et al.
**摘要**：研究报道了针对人源AST的单克隆抗体制备及特性分析，通过杂交瘤技术成功获得高特异性抗体，并验证其在ELISA和免疫印迹中的应用，为临床检测AST水平提供可靠工具。
2. **文献名称**：*"Immunoassay development for the detection of liver injury using AST-specific antibodies"*
**作者**：Chen L, et al.
**摘要**：开发了一种基于AST抗体的新型免疫检测方法，用于评估肝损伤程度。实验证明，该抗体在血清样本中能灵敏区分AST同工酶(线粒体型和胞浆型)，显著提高肝病诊断特异性。
3. **文献名称**：*"Cross-reactivity of anti-AST antibodies in murine models of myocardial infarction"*
**作者**：Yamamoto K, et al.
**摘要**：探讨了商业抗小鼠AST抗体在心肌梗死模型中的交叉反应性，发现部分抗体与心肌细胞损伤标志物存在交叉反应，提示需优化抗体选择以避免假阳性结果。
4. **文献名称**：*"Role of anti-mitochondrial AST antibodies in autoimmune hepatitis diagnosis"*
**作者**：Rodriguez M, et al.
**摘要**：分析了线粒体型AST抗体在自身免疫性肝炎中的诊断价值，发现其与疾病活动度相关，可能作为辅助生物标志物用于鉴别诊断和疗效监测。
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以上文献涵盖抗体开发、检测方法优化、交叉反应验证及临床诊断应用，反映AST抗体在基础研究与临床实践中的多样性用途。
Aspartate aminotransferase (AST), also known as glutamic oxaloacetic transaminase (GOT), is a key enzyme involved in amino acid metabolism, catalyzing the transfer of an amino group between aspartate and alpha-ketoglutarate. It is primarily found in the liver, heart, skeletal muscle, and kidneys, with elevated serum levels serving as a biomarker for tissue damage, particularly in liver or cardiac diseases. AST antibodies are immunodetection tools designed to specifically recognize and bind to AST protein isoforms (AST1 and AST2) in research or diagnostic applications. These antibodies are typically produced in host species like rabbits or mice using purified AST protein or peptide antigens.
In research, AST antibodies are utilized in techniques such as Western blotting, immunohistochemistry (IHC), and ELISA to study enzyme expression, localization, and regulation in tissues or cell cultures. They help investigate AST's role in metabolic disorders, drug-induced hepatotoxicity, or myocardial infarction. Commercially available AST antibodies are often validated for specificity and cross-reactivity across species (e.g., human, mouse, rat) to ensure reliability. Quality control includes testing for minimal cross-reactivity with related enzymes like alanine aminotransferase (ALT). Their applications extend to developing diagnostic assays and exploring therapeutic targets in diseases involving mitochondrial dysfunction or cellular stress responses.
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