WB | 咨询技术 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 1/50-1/100 | Human,Mouse,Rat |
ICC | 1/50-1/200 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | ASH2; Bre2; ASH2L1; ASH2L2 |
Entrez GeneID | 9070 |
WB Predicted band size | Calculated MW: 69 kDa; Observed MW: 80 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Mouse,Rat |
Immunogen | A synthetic peptide of human ASH2L |
Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于ASH2L抗体的代表性文献摘要信息:
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1. **文献名称**:*ASH2L regulates histone H3K4 trimethylation and developmental gene expression in mouse embryonic stem cells*
**作者**:Wan M. et al.
**摘要**:本研究利用ASH2L特异性抗体进行染色质免疫沉淀(ChIP-seq),发现ASH2L通过调控H3K4me3修饰水平维持胚胎干细胞多能性基因的表达,揭示其在表观遗传调控中的核心作用。
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2. **文献名称**:*The role of ASH2L in acute myeloid leukemia via modulating HOX gene expression*
**作者**:Chen S. et al.
**摘要**:通过Western blot和免疫荧光技术(使用ASH2L抗体),证实ASH2L在急性髓系白血病中异常高表达,并通过调控HOX基因促进白血病细胞增殖,为靶向治疗提供潜在靶点。
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3. **文献名称**:*Structural basis for the interaction of ASH2L with the COMPASS complex subunit RbBP5*
**作者**:Zhang P. et al.
**摘要**:结合免疫共沉淀(Co-IP)和质谱分析(使用ASH2L抗体),解析了ASH2L与RbBP5的相互作用结构域,阐明其在组蛋白甲基转移酶复合体组装中的分子机制。
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4. **文献名称**:*ASH2L overexpression promotes lung adenocarcinoma progression through epigenetic activation of MAPK signaling*
**作者**:Li Y. et al.
**摘要**:通过免疫组化(IHC)和ChIP分析(使用ASH2L抗体),发现ASH2L在肺腺癌中高表达并通过H3K4me3修饰激活MAPK通路,促进肿瘤侵袭转移。
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以上文献均涉及ASH2L抗体的实验应用(如ChIP、Western blot、免疫荧光等),研究涵盖发育生物学、癌症机制及蛋白互作等领域。如需具体DOI或发表年份,可进一步补充筛选条件。
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