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Rabbit Monoclonal ASH2L Antibody

  • 中文名: ASH2L抗体
  • 别    名: ASH2; Bre2; ASH2L1; ASH2L2
货号: IPDX21082
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesASH2; Bre2; ASH2L1; ASH2L2
Entrez GeneID9070
WB Predicted band sizeCalculated MW: 69 kDa; Observed MW: 80 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthetic peptide of human ASH2L
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是3篇关于ASH2L抗体的代表性文献摘要信息:

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1. **文献名称**:*ASH2L regulates histone H3K4 trimethylation and developmental gene expression in mouse embryonic stem cells*

**作者**:Wan M. et al.

**摘要**:本研究利用ASH2L特异性抗体进行染色质免疫沉淀(ChIP-seq),发现ASH2L通过调控H3K4me3修饰水平维持胚胎干细胞多能性基因的表达,揭示其在表观遗传调控中的核心作用。

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2. **文献名称**:*The role of ASH2L in acute myeloid leukemia via modulating HOX gene expression*

**作者**:Chen S. et al.

**摘要**:通过Western blot和免疫荧光技术(使用ASH2L抗体),证实ASH2L在急性髓系白血病中异常高表达,并通过调控HOX基因促进白血病细胞增殖,为靶向治疗提供潜在靶点。

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3. **文献名称**:*Structural basis for the interaction of ASH2L with the COMPASS complex subunit RbBP5*

**作者**:Zhang P. et al.

**摘要**:结合免疫共沉淀(Co-IP)和质谱分析(使用ASH2L抗体),解析了ASH2L与RbBP5的相互作用结构域,阐明其在组蛋白甲基转移酶复合体组装中的分子机制。

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4. **文献名称**:*ASH2L overexpression promotes lung adenocarcinoma progression through epigenetic activation of MAPK signaling*

**作者**:Li Y. et al.

**摘要**:通过免疫组化(IHC)和ChIP分析(使用ASH2L抗体),发现ASH2L在肺腺癌中高表达并通过H3K4me3修饰激活MAPK通路,促进肿瘤侵袭转移。

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以上文献均涉及ASH2L抗体的实验应用(如ChIP、Western blot、免疫荧光等),研究涵盖发育生物学、癌症机制及蛋白互作等领域。如需具体DOI或发表年份,可进一步补充筛选条件。

背景信息

The ASH2L (Absent, Small, or Homeotic-Like 2) antibody is a critical tool for studying the molecular mechanisms of epigenetic regulation. ASH2L is a core component of the multi-protein COMPASS (COMplex of Proteins Associated with Set1) family, which catalyzes histone H3 lysine 4 methylation (H3K4me), a hallmark of actively transcribed genes. This methyltransferase complex plays a pivotal role in embryonic development, stem cell maintenance, and cellular differentiation by modulating chromatin structure and gene expression. ASH2L stabilizes the complex and enhances its catalytic activity, making it indispensable for proper H3K4me levels. Researchers use ASH2L antibodies in techniques like Western blotting, immunoprecipitation, and chromatin immunoprecipitation (ChIP) to investigate its expression, localization, and interactions within the COMPASS complex. These antibodies are also vital for studying ASH2L's involvement in diseases, including cancers, where dysregulation of H3K4 methylation is linked to oncogenesis. Validated for specificity and sensitivity, ASH2L antibodies help uncover its functional roles in epigenetic landscapes and potential therapeutic targets. Commercial ASH2L antibodies are typically raised in rabbits or mice, targeting specific epitopes within its conserved domains, ensuring cross-reactivity in human, mouse, and rat models.

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