| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MAP3K5; ASK1; MAPKKK5; MEKK5; Mitogen-activated protein kinase kinase kinase 5; Apoptosis signal-regulating kinase 1; ASK-1; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5 |
| Entrez GeneID | 4217 |
| WB Predicted band size | Calculated MW: 155 kDa; Observed MW: 155 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human ASK1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于ASK1抗体的3篇参考文献，包含文献名称、作者及摘要概括：
1. **文献名称**: *"ASK1 is essential for endoplasmic reticulum stress-induced neuronal cell death triggered by expanded polyglutamine repeats"*
**作者**: H. Nishitoh et al.
**摘要**: 该研究通过Western blot和免疫组化使用ASK1抗体，发现内质网应激通过激活ASK1-p38通路介导多聚谷氨酰胺重复扩张引起的神经元凋亡，揭示了ASK1在神经退行性疾病中的作用机制。
2. **文献名称**: *"Targeted deletion of apoptosis signal-regulating kinase 1 attenuates left ventricular remodeling after myocardial infarction in mice"*
**作者**: T. Izumiya et al.
**摘要**: 利用ASK1敲除小鼠及特异性抗体检测，证明ASK1促进心肌梗死后左心室纤维化及炎症反应，抑制ASK1可改善心脏重构，为心血管疾病治疗提供新靶点。
3. **文献名称**: *"The ASK1-MAP kinase signaling in ER stress and neurodegenerative diseases"*
**作者**: S. Matsukawa et al.
**摘要**: 该综述总结了ASK1抗体在检测内质网应激及神经退行性疾病模型中的应用，强调ASK1-MAPK通路在阿尔茨海默病和帕金森病中的调控作用。
以上文献均明确使用ASK1抗体进行蛋白表达及活性检测，涉及神经、心血管疾病及分子机制研究。
The apoptosis signal-regulating kinase 1 (ASK1) antibody is a crucial tool for studying the ASK1 protein, a serine/threonine kinase central to stress-activated signaling pathways. ASK1. also known as MAP3K5. belongs to the mitogen-activated protein kinase kinase kinase (MAP3K) family. It activates downstream kinases MKK4/7 and MKK3/6. which in turn regulate JNK and p38 MAPK pathways involved in apoptosis, inflammation, and cellular responses to oxidative stress, endoplasmic reticulum (ER) stress, or cytokine stimulation. Dysregulation of ASK1 is linked to diseases such as cancer, neurodegenerative disorders, and cardiovascular conditions.
ASK1 antibodies are widely used in research to detect ASK1 expression, phosphorylation status, and interactions in immunoblotting (Western blot), immunoprecipitation, or immunohistochemistry. Specific antibodies may target distinct regions, such as the N-terminal inhibitory domain, C-terminal kinase domain, or phosphorylated residues (e.g., Thr845 in humans), enabling analysis of ASK1 activation. For example, phosphorylated ASK1 antibodies help assess its activation during stress responses, while total ASK1 antibodies measure overall protein levels. These reagents are vital for elucidating ASK1's regulatory mechanisms, including its inhibition by thioredoxin under normal conditions or activation via TRAF2/6 in inflammatory signaling. Commercial ASK1 antibodies are often validated for species reactivity (human, mouse, rat) and application-specific performance.
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