首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人三叶因子2(TFF2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、组织培养上清液样本中的TFF2含量。TFF2即三叶因子2，它是一种具有三叶结构的小分子蛋白，在胃肠道黏膜保护与修复等方面起着重要作用。研究机制上，主要聚焦于它如何促进细胞迁移、增殖，抵抗凋亡，调节黏液合成与分泌，以维持胃肠道黏膜的完整性和稳定性。试剂盒检测范围为31.25 ng/mL-2000 ng/mL，灵敏度为7.81 ng/mL。本试剂盒适用于基础医学研究中TFF2的生物学功能探索，包括黏膜修复机制、炎症调控及肿瘤微环境研究，可配合多种样本类型灵活应用：血清/血浆用于评估循环系统中TFF2的动态变化，组织匀浆适用于组织局部表达量分析，而细胞培养上清则为体外实验模型提供便捷检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：SML 1 ELISA Kit; SML1 ELISA Kit; SP ELISA Kit; Spasmolysin ELISA Kit; Spasmolytic polypeptide ELISA Kit; spasmolytic protein 1 ELISA Kit; TFF 2 ELISA Kit; TFF2 ELISA Kit; TFF2_HUMAN ELISA Kit; trefoil factor 2 (spasmolytic protein 1) ELISA Kit; Trefoil factor 2 ELISA Kit; Trefoil factor 2 precursor ELISA Kit
缩写：TFF2
Uniprot No.：Q03403
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell culture supernates
检测范围：31.25 ng/mL-2000 ng/mL
灵敏度：7.81 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TFF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 90-103 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 88-97 | |||||
回收率：
| The recovery of human TFF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 98 | 90-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected20002.0262.0072.0171.92010001.3641.3061.3351.2385000.7880.7240.7560.6592500.4570.4270.4420.3451250.2620.2460.2540.15762.50.1750.1670.1710.07431.250.1190.1140.1170.02000.1010.0930.097 | ||||||
货期：3-5 working days
×
