首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠组织因子途径抑制物(TFPI)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的TFPI含量。TFPI即组织因子途径抑制物，是一种天然存在的抗凝蛋白。它能抑制组织因子（TF）介导的凝血途径，通过与因子Xa和TF - 因子VIIa复合物结合，发挥抗凝作用。其在调节凝血平衡中起关键作用，对其研究有助于开发新型抗凝药物等。试剂盒检测范围为7.8 ng/mL-500 ng/mL，为研究凝血机制、心血管疾病模型、药物干预实验及炎症相关研究提供可靠工具，特别适用于评估凝血相关因子调控、病理模型建立及治疗性化合物筛选等科研场景，所有检测结果仅用于科学研究目的。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tfpi ELISA Kit; Tissue factor pathway inhibitor ELISA Kit; TFPI ELISA Kit; Extrinsic pathway inhibitor ELISA Kit; EPI ELISA Kit; Lipoprotein-associated coagulation inhibitor ELISA Kit; LACI ELISA Kit
缩写：TFPI
Uniprot No.：Q02445
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：7.8 ng/mL-500 ng/mL
灵敏度：1.95 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TFPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 85-96 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 84-95 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 85-94 | |||||
回收率：
| The recovery of rat TFPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 95-107 | ||||
| EDTA plasma (n=4) | 92 | 88-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 5002.5962.4742.5352.424 2501.9781.9021.9401.829 1251.1721.0961.1341.023 62.50.6870.7020.6950.584 31.20.4010.4240.4130.302 15.60.2820.2910.2870.176 7.80.1820.1750.1790.068 00.1130.1080.111 | ||||||
货期：3-5 working days
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