首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠凝血酶抗凝血酶复合物(TAT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的TAT含量。大鼠凝血酶抗凝血酶复合物（TAT）是凝血酶与抗凝血酶结合形成的复合物，反映凝血酶生成和活性，用于评估抗凝药物效果、研究血栓形成机制及血液疾病诊断。通过ELISA技术检测TAT水平，可监测机体凝血状态和血栓前状态。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于科研场景中动态监测凝血激活状态，如在心血管疾病模型、血栓性疾病动物实验或药物干预研究中分析凝血与抗凝血平衡，也可用于体外细胞实验中探究凝血相关信号通路或药物对凝血级联反应的影响。其宽泛的样本兼容性支持从基础研究到药物开发的多层次实验需求，为凝血功能相关研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：TAT
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:10 | Average % | 103 | ||||
| Range % | 97-109 | |||||
| 1:20 | Average % | 95 | ||||
| Range % | 90-98 | |||||
| 1:40 | Average % | 96 | ||||
| Range % | 90-102 | |||||
回收率：
| The recovery of rat TAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 82-94 | ||||
| EDTA plasma (n=4) | 94 | 87-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.5042.3852.4452.32410001.7121.6331.6731.5525001.0101.0011.0060.8852500.6060.6250.6160.4951250.3870.3960.3920.27162.50.2890.2950.2920.17131.250.1930.1960.1950.07400.1200.1210.121 | ||||||
货期：3-5 working days
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