首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔凝血酶抗凝血酶复合物(TAT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的TAT含量。TAT（转录激活因子）是HIV-1病毒的关键调控蛋白，其在病毒复制和致病过程中发挥重要作用。TAT通过稳定Tat-TAR复合体增强HIV-1转录活性，调控T细胞免疫应答。研究发现，TAT与宿主免疫应答通路相互作用，为HIV治疗提供了新的分子靶点。试剂盒检测范围为3.12 ng/mL-200 ng/mL，该产品适用于科研领域对兔模型凝血功能的研究，如血栓形成机制探索、抗凝药物疗效评估或心血管疾病动物模型中凝血激活程度的动态监测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：TAT
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 91-100 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 85-98 | |||||
| 1:4 | Average % | 90-105 | ||||
| Range % | 83 | |||||
| 1:8 | Average % | 81-92 | ||||
| Range % | 96 | |||||
回收率：
| The recovery of rabbit TAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 84-98 | ||||
| EDTA plasma (n=4) | 91 | 85-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected2002.5172.4202.4692.2321002.1512.0762.1141.877501.8011.7041.7531.516251.4521.3391.3961.15912.51.0321.0241.0280.7916.250.8310.7790.8050.5683.120.4140.4090.4120.17500.2450.2280.237 | ||||||
货期：3-5 working days
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