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产品描述：猪凝血酶抗凝血酶复合物(TAT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TAT含量。猪凝血酶抗凝血酶复合物（TAT）是凝血酶与抗凝血酶Ⅲ（ATⅢ）结合形成的复合物，可作为凝血酶生成的分子标志物，反映凝血系统活化，对血栓形成和凝血-抗凝血平衡有重要诊断意义。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于实验室开展动物凝血机制研究、血栓性疾病模型评估、抗凝药物效果分析等科研方向，可为心血管疾病、术后凝血监测等基础研究提供标准化检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：TAT
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig TAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 84-100 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-108 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 82-105 | |||||
回收率：
| The recovery of pig TAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 85-102 | ||||
| EDTA plasma (n=4) | 98 | 92-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.1782.0282.1031.89720001.5821.5601.5711.36510000.9800.9490.9650.7595000.5580.5650.5620.3562500.3700.3470.3590.1531250.2960.3020.2990.09362.50.2650.2750.2700.06400.2000.2120.206 | ||||||
货期：3-5 working days
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