首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠凝血酶抗凝血酶复合物(TAT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TAT含量。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，常用于评估凝血激活状态及相关病理机制研究；适用于小鼠模型的血栓性疾病、弥散性血管内凝血（DIC）等凝血功能障碍研究，或药物干预下凝血/抗凝血系统调控机制的体外实验分析；为心血管疾病、炎症反应或肿瘤相关凝血异常的动物模型研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：TAT
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:10 | Average % | 87 | ||||
| Range % | 82-93 | |||||
| 1:20 | Average % | 95 | ||||
| Range % | 90-101 | |||||
| 1:40 | Average % | 105 | ||||
| Range % | 96-112 | |||||
| 1:80 | Average % | 93 | ||||
| Range % | 87-98 | |||||
回收率：
| The recovery of mouse TAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 93-100 | ||||
| EDTA plasma (n=4) | 90 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.6582.4562.5572.374 5002.2142.1132.1641.981 2501.5031.5221.5131.330 1250.8550.9040.8800.697 62.50.5420.5630.5530.370 31.20.4130.4350.4240.241 15.60.2910.3020.2970.114 00.1880.1780.183 | ||||||
货期：3-5 working days
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