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产品描述:人丝甘蛋白聚糖(SRGN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的SRGN含量。SRGN即聚硫蛋白聚糖,在多种生理和病理过程中发挥作用。它参与细胞黏附、迁移等过程,还与炎症反应相关。研究发现其在肿瘤微环境中可调节免疫细胞功能,影响肿瘤进展,目前是肿瘤及免疫相关研究中值得关注的靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL,适用于探索SRGN在免疫细胞功能研究、肿瘤微环境分析、炎症性疾病模型构建等科研场景中的表达特征与调控机制,为深入解析SRGN在疾病发生发展中的分子机制提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:Chondroitin sulfate proteoglycan core protein ELISA Kit; Cytolytic granule proteoglycan core protein ELISA Kit; FLJ12930 ELISA Kit; gp600 ELISA Kit; Hematopoetic proteoglycan core protein ELISA Kit; Mastocytoma proteoglycan core protein ELISA Kit; MGC9289 ELISA Kit; OTTHUMP00000019716 ELISA Kit; P.PG ELISA Kit; PG19 core protein ELISA Kit; Pgsg ELISA Kit; Platelet proteoglycan core protein ELISA Kit; platelet proteoglycan protein core ELISA Kit; PLATELET PROTEOGLYCAN PROTEIN CORE; PPG ELISA Kit; PPG ELISA Kit; PRG ELISA Kit; PRG1 ELISA Kit; PROTEOGLYCAN 1 ELISA Kit; proteoglycan 1; secretory granule ELISA Kit; Proteoglycan 10K core protein ELISA Kit; Proteoglycan peptide core protein ELISA Kit; PROTEOGLYCAN PROTEIN CORE FOR MAST CELL SECRETORY GRANULE ELISA Kit; secretory granule proteoglycan 1 ELISA Kit; Secretory granule proteoglycan core peptide ELISA Kit; Secretory granule proteoglycan core protein ELISA Kit; Serglycin ELISA Kit; serglycin proteoglycan ELISA Kit; Sgc ELISA Kit; Srgn ELISA Kit; SRGN_HUMAN ELISA Kit
缩写:SRGN
Uniprot No.:P10124
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:0.312 ng/mL-20 ng/mL
灵敏度:0.078 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Cell Biology
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human SRGN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 107 | |||||
Range % | 103-112 | ||||||
1:2 | Average % | 97 | |||||
Range % | 93-102 | ||||||
1:4 | Average % | 94 | |||||
Range % | 88-98 | ||||||
1:8 | Average % | 96 | |||||
Range % | 92-99 |
回收率:
The recovery of human SRGN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 93 | 88-97 | |||||
EDTA plasma (n=4) | 87 | 82-91 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
ng/mlOD1OD2AverageCorrected202.6122.5352.5742.414102.2652.1712.2182.05851.6671.6321.6501.4902.51.0781.0641.0710.9111.250.6750.6690.6720.5120.6250.4430.4380.4410.2810.3120.3280.3230.3260.16600.1610.1590.160 |
货期:3-5 working days
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