首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠固醇调节元件结合蛋白-1C(SREBP-1C)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SREBP-1C含量。SREBP-1C是一种重要靶点。它在脂质代谢中扮演关键角色，能调节脂肪酸、甘油三酯等合成相关基因表达。对其研究主要集中在揭示其如何调控脂质合成信号通路，以及在代谢性疾病如肥胖、脂肪肝等发病机制中的作用。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于探究脂代谢通路调控、肝脏脂肪变性机制、营养干预或药物对脂质合成的影响等科研场景；为体外研究SREBP - 1C在能量稳态及代谢紊乱中的作用提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Srebf1; Srebp1; Sterol regulatory element-binding protein 1; SREBP-1; Adipocyte determination- and differentiation-dependent factor 1; ADD1; Sterol regulatory element-binding transcription factor 1
缩写：SREBP-1C
Uniprot No.：P56720
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat SREBP-1C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 90 | ||||
| Range % | 86-96 | |||||
| 1:10 | Average % | 101 | ||||
| Range % | 95-108 | |||||
| 1:20 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:40 | Average % | 92 | ||||
| Range % | 87-96 | |||||
回收率：
| The recovery of rat SREBP-1C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 90-96 | ||||
| EDTA plasma (n=4) | 96 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.5382.5692.5542.35620001.8981.8661.8821.68410001.3811.3511.3661.1685000.9590.9990.9790.7812500.5740.5870.5810.3831250.3640.3810.3730.17562.50.3040.3280.3160.11800.1960.2000.198 | ||||||
货期：3-5 working days
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