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产品描述：大鼠3氧代5α类固醇4脱氢酶2(SRD5A2)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的SRD5A2含量。SRD5A2 即 II 型 5α-还原酶，它能将睾酮转化为二氢睾酮。该靶点与良性前列腺增生、雄激素性脱发等疾病密切相关。研究机制主要聚焦在研发其抑制剂，以减少二氢睾酮生成，改善相关病症，相关药物已在临床应用。试剂盒检测范围为6 pg/mL-1200 pg/mL，适用于探索大鼠模型中SRD5A2的表达水平与雄激素相关生理病理过程的关联性，例如生殖内分泌机制研究、前列腺组织代谢分析或药物干预对类固醇合成途径的影响评估等本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Srd5a2; 3-oxo-5-alpha-steroid 4-dehydrogenase 2; 5 alpha-SR2; SR type 2; Steroid 5-alpha-reductase 2; S5AR 2
缩写：SRD5A2
Uniprot No.：P31214
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：6 pg/mL-1200 pg/mL
灵敏度：3 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat SRD5A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 89-99 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 90-103 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 87-94 | |||||
回收率：
| The recovery of rat SRD5A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 95-101 | ||||
| EDTA plasma (n=4) | 93 | 87-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 12000.2250.2170.221 3000.4110.4340.423 960.6510.6320.642 241.0551.0171.036 61.4971.4581.478 02.3592.4502.405 | ||||||
货期：3-5 working days
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