首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人固醇调节因子结合蛋白1(SREBF1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的SREBF1含量。SREBF1是一种重要的靶点。它在细胞内脂质代谢调控中起关键作用。相关研究围绕其调控机制展开，揭示它能激活下游脂质合成基因转录。对其深入研究有助于理解脂代谢紊乱疾病发病机制，为开发针对性治疗药物提供理论基础。试剂盒检测范围为7.8 pg/mL-500 pg/mL，适用于基础科研领域，如代谢性疾病分子机制研究、药物干预效果评估或细胞模型中脂代谢通路的动态监测；科研人员可通过高效获取SREBF1蛋白在心血管疾病模型、肝脏代谢研究或肥胖相关实验中的定量表达数据，为探索脂质代谢调控网络提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：SREBF1; BHLHD1; SREBP1; Sterol regulatory element-binding protein 1; SREBP-1; Class D basic helix-loop-helix protein 1; bHLHd1; Sterol regulatory element-binding transcription factor 1
缩写：SREBF1
Uniprot No.：P36956
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SREBF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 92 | |||||
| Range % | 88-99 | ||||||
| 1:2 | Average % | 90 | |||||
| Range % | 85-97 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 90-100 | ||||||
| 1:8 | Average % | 94 | |||||
| Range % | 86-102 | ||||||
回收率：
| The recovery of human SREBF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 96 | 91-98 | |||||
| EDTA plasma (n=4) | 97 | 92-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected5002.6172.6582.6382.5322502.2462.2732.2602.1541251.7861.7901.7881.68262.51.1751.1991.1871.08131.20.6380.6390.6390.53315.60.3690.3550.3620.2567.80.1960.2070.2020.09600.1050.1060.106 | |||||||
货期：3-5 working days
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