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产品描述：人3氧代5α类固醇4脱氢酶2(SRD5A2)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SRD5A2含量。SRD5A2是重要靶点，它能催化睾酮转化为活性更强的双氢睾酮。在人体生理过程如前列腺发育、雄激素性脱发等方面起关键作用。相关研究围绕其抑制机制开展，旨在研发针对性药物，用于治疗前列腺增生、脱发等疾病。试剂盒检测范围为12 pg/mL-2400 pg/mL，适用于实验室环境下探究SRD5A2在激素代谢通路、前列腺组织生理病理过程或雄性特征调控中的表达特征，为生殖内分泌、肿瘤微环境等基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：SRD5A2; 3-oxo-5-alpha-steroid 4-dehydrogenase 2; 5 alpha-SR2; SR type 2; Steroid 5-alpha-reductase 2; S5AR 2; Type II 5-alpha reductase
缩写：SRD5A2
Uniprot No.：P31213
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：12 pg/mL-2400 pg/mL
灵敏度：6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SRD5A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 85-93 | |||||
| 1:2 | Average % | 91 | ||||
| Range % | 87-98 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-99 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 82-97 | |||||
回收率：
| The recovery of human SRD5A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-102 | ||||
| EDTA plasma (n=4) | 97 | 91-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 02.0532.1372.095 121.4981.4881.493 481.0861.0521.069 1920.5910.6200.606 6000.2610.2760.269 24000.1140.1150.115 | ||||||
货期：3-5 working days
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