首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠类固醇5α还原酶1 (SRD5A1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的SRD5A1含量。SRD5A1即5α-还原酶1型，在多种组织中表达。它能将睾酮转化为活性更强的双氢睾酮。对其研究机制主要围绕调节雄激素活性展开，在前列腺疾病、脱发等病症的发生发展中扮演角色，为相关治疗药物研发提供了重要靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于实验室研究中对SRD5A1表达水平的动态监测，如内分泌代谢研究、药物干预实验或疾病模型构建中酶活性变化的定量分析。为探索SRD5A1在生理及病理过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Srd5a1; 3-oxo-5-alpha-steroid 4-dehydrogenase 1; SR type 1; Steroid 5-alpha-reductase 1; S5AR 1
缩写：SRD5A1
Uniprot No.：P24008
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat SRD5A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 100 | ||||
| Range % | 95-105 | |||||
| 1:10 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:20 | Average % | 94 | ||||
| Range % | 88-99 | |||||
| 1:40 | Average % | 90 | ||||
| Range % | 85-95 | |||||
回收率：
| The recovery of rat SRD5A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 101 | 98-105 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.7622.8032.7832.5895002.1172.1042.1111.9172501.5981.5831.5911.3971251.1031.1361.1200.92662.50.6520.6720.6620.46831.250.4160.4260.4210.22715.60.3410.3510.3460.15200.1930.1950.194 | ||||||
×
