| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NEDD8; NEDD8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; NEDD-8; Ubiquitin-like protein Nedd8 |
| Entrez GeneID | 4738 |
| WB Predicted band size | Calculated MW: 9 kDa; Observed MW: 9 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human NEDD8 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于NEDD8抗体的3篇参考文献示例(信息基于公开文献概括，非真实引用)：
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1. **"A highly specific NEDD8 antibody distinguishes ubiquitin and NEDD8 modifications in cellular pathways"**
*Authors: Smith J, et al.*
摘要：开发了一种高特异性NEDD8单克隆抗体，可区分NEDD8与泛素的共价修饰，验证了其在免疫印迹和免疫荧光中的应用，并揭示NEDDylation在肿瘤细胞中的动态调控机制。
2. **"NEDD8 conjugation in Alzheimer's disease: Detection using a novel monoclonal antibody"**
*Authors: Lee H, et al.*
摘要：报道了一种新型NEDD8抗体的开发，用于检测阿尔茨海默病模型中的异常蛋白质NEDDylation，发现其与tau蛋白病理沉积的相关性，为神经退行性疾病研究提供工具。
3. **"Targeting neddylation with a small-molecule inhibitor and antibody-based approach in cancer therapy"**
*Authors: Chen X, et al.*
摘要：结合NEDD8特异性抗体与小分子抑制剂MLN4924.系统研究neddylation通路在癌症中的功能，证明抗体在体内外模型中可有效监测药物疗效及靶标蛋白修饰水平。
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注：以上为模拟示例，实际文献需通过PubMed/Google Scholar等平台检索关键词(如"NEDD8 antibody" "neddylation detection")获取真实数据。
The NEDD8 antibody is a critical tool for studying the post-translational modification process called neddylation, where the ubiquitin-like protein NEDD8 (Neural Precursor Cell Expressed Developmentally Downregulated 8) is covalently conjugated to target proteins. NEDD8 shares ~60% sequence identity with ubiquitin but has distinct biological functions. Its primary role is to regulate the activity of Cullin-RING ligases (CRLs), a major class of E3 ubiquitin ligases that control protein degradation. By modifying Cullin proteins, NEDD8 activates CRLs, enabling their participation in ubiquitination cascades that influence cell cycle progression, DNA repair, and stress responses.
NEDD8 antibodies are widely used to detect neddylated proteins in techniques like Western blotting, immunofluorescence, and immunoprecipitation. Specificity is crucial, as cross-reactivity with ubiquitin or other ubiquitin-like proteins (e.g., SUMO) can lead to false interpretations. High-quality antibodies differentiate between free Nedd8. conjugated Nedd8. and related proteins.
Research involving NEDD8 antibodies has expanded into cancer biology, as dysregulated neddylation is linked to tumorigenesis. Inhibitors targeting the NEDD8 pathway (e.g., MLN4924/Pevonedistat), which block CRL activation, are under clinical investigation as anticancer therapies. These antibodies help validate drug efficacy by monitoring neddylation suppression in preclinical models.
Additionally, NEDD8 antibodies aid in studying neurodegenerative diseases and viral infections, where protein homeostasis imbalances occur. Their versatility makes them indispensable for both mechanistic studies and therapeutic development, bridging molecular insights with clinical applications.
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