首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠胞外铜锌超氧化物歧化酶(Cu/Zn-SOD/SOD3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的SOD3含量。SOD3即超氧化物歧化酶 3，它是一种细胞外抗氧化酶，能将超氧阴离子转化为过氧化氢和氧气。在研究机制方面，其在多种疾病如心血管疾病、肺部疾病等中发挥作用，可调节氧化应激水平，有望成为治疗相关疾病的潜在靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于研究氧化应激相关疾病模型、药物干预对SOD3活性的影响，以及细胞或组织微环境中抗氧化机制的动态变化。可满足神经退行性疾病、心血管疾病、炎症反应等研究领域中对SOD3蛋白表达水平的定量分析需求，为探索氧化还原平衡调控机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Sod3 ELISA Kit; Sod-3 ELISA Kit; Extracellular superoxide dismutase [Cu-Zn] ELISA Kit; EC-SOD ELISA Kit; EC 1.15.1.1 ELISA Kit; Superoxide dismutase B ELISA Kit
缩写：SOD3
Uniprot No.：Q08420
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Cu/Zn-SOD/SOD3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 93 | ||||
| Range % | 89-95 | |||||
| 1:400 | Average % | 104 | ||||
| Range % | 100-107 | |||||
| 1:800 | Average % | 95 | ||||
| Range % | 90-97 | |||||
| 1:1600 | Average % | 98 | ||||
| Range % | 91-101 | |||||
回收率：
| The recovery of rat Cu/Zn-SOD/SOD3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-97 | ||||
| EDTA plasma (n=4) | 95 | 92-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.6442.5882.6162.461 5002.2642.3442.3042.149 2501.6501.6321.6411.486 1251.0441.0281.0360.881 62.50.6240.6360.6300.475 31.20.3830.3680.3760.221 15.60.2480.2390.2440.089 00.1560.1540.155 | ||||||
货期：3-5 working days
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