| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | SOX9; Transcription factor SOX-9 |
| Entrez GeneID | 6662 |
| WB Predicted band size | Calculated MW: 56 kDa; Observed MW: 70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Recombinant protein of human SOX9 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于SOX9抗体的3篇参考文献示例(文献信息为模拟示例，仅供参考)：
1. **文献名称**：SOX9 Antibody Validation in Chondrocyte Differentiation Models
**作者**：Smith A, et al.
**摘要**：研究验证了多种SOX9抗体在软骨细胞分化模型中的特异性，发现部分抗体存在交叉反应性，推荐使用克隆号XX的抗体用于软骨发育研究。
2. **文献名称**：Role of SOX9 in Pancreatic Cancer: An Immunohistochemical Analysis
**作者**：Zhang L, et al.
**摘要**：通过免疫组化技术，使用SOX9抗体检测其在胰腺癌组织中的表达，发现SOX9高表达与患者预后不良显著相关。
3. **文献名称**：Comparative Study of Commercial SOX9 Antibodies for Stem Cell Research
**作者**：Brown K, et al.
**摘要**：对比了5种市售SOX9抗体在人多能干细胞中的染色效果，确定了抗体Y在Western blot和免疫荧光中的最优表现。
(注：以上为模拟内容，实际文献需通过PubMed、Google Scholar等平台检索。)
SOX9 (SRY-box transcription factor 9) is a member of the SOX protein family, characterized by a high-mobility group (HMG) DNA-binding domain. It plays pivotal roles in embryonic development, including chondrogenesis, male sex determination, and organogenesis. SOX9 regulates target gene expression by binding to specific DNA sequences, often in collaboration with other transcription factors. Dysregulation of SOX9 is linked to developmental disorders (e.g., campomelic dysplasia) and diseases such as cancer, where it may act as an oncogene or tumor suppressor depending on context.
SOX9 antibodies are critical tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). These antibodies typically target conserved regions, such as the N-terminal transactivation domain or C-terminal regions, enabling detection across species (human, mouse, rat). Validation via knockout controls or siRNA knockdown is essential to confirm specificity.
In research, SOX9 antibodies help investigate its role in stem cell maintenance, tissue repair, and cancer progression (e.g., glioblastoma, prostate, and colorectal cancers). They also aid in diagnosing SOX9-related pathologies and evaluating therapeutic targets. Commercial availability from multiple suppliers ensures broad accessibility, though batch-to-batch variability requires careful experimental optimization.
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