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Mouse Extracellular superoxide dismutase [Cu-Zn](SOD3) ELISA kit

  • 中文名: 小鼠胞外超氧化物歧化酶[Cu-Zn](SOD3)ELISA kit
货号: IPD-EL022399MO
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    小鼠胞外超氧化物歧化酶[Cu-Zn](SOD3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的SOD3含量。SOD3(超氧化物歧化酶 3)是一种重要的抗氧化酶,主要存在于细胞外。它可催化超氧阴离子转化为过氧化氢和氧气,减少体内氧化应激。目前其在心血管疾病、炎症等方面的研究较多,通过调节氧化还原状态,影响细胞信号传导与功能。试剂盒检测范围为7.8 pg/mL-500 pg/mL,适用于实验室研究SOD3在氧化应激相关疾病模型中的表达调控、药物干预效果评估,或用于探索其在代谢综合征、神经退行性疾病中的分子机制,为生命科学基础研究提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    Sod3 ELISA Kit; Extracellular superoxide dismutase [Cu-Zn] ELISA Kit; EC-SOD ELISA Kit; EC 1.15.1.1 ELISA Kit
  • 缩写:
    SOD3
  • Uniprot No.:
    O09164
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    7.8 pg/mL-500 pg/mL
  • 灵敏度:
    1.95 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Metabolism
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse SOD3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:500

    Average %

    89


    Range %

    84-94


    1:1000

    Average %

    96


    Range %

    89-103


    1:2000

    Average %

    93


    Range %

    88-97


    1:4000

    Average %

    88


    Range %

    83-93


  • 回收率:

    The recovery of mouse SOD3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    94

    89-99


    EDTA plasma (n=4)

    90

    86-96


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    500

    2.469

    2.634

    2.551

    2.374


    250

    1.659

    1.667

    1.663

    1.485


    125

    1.159

    1.098

    1.128

    0.951


    62.5

    0.712

    0.729

    0.720

    0.543


    31.2

    0.468

    0.471

    0.470

    0.292


    15.6

    0.328

    0.333

    0.330

    0.153


    7.8

    0.261

    0.274

    0.267

    0.090


    0

    0.180

    0.175

    0.178




  • 货期:
    3-5 working days

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