| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | p16 Arc; ARPC5; ARC16; Arp2/3 protein complex subunit p16 |
| Entrez GeneID | 10092 |
| WB Predicted band size | Calculated MW: 16 kDa; Observed MW: 16 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human p16 ARC |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于p16抗体(ARC抗体常用于免疫组化检测)的参考文献及摘要概括：
1. **文献名称**：*p16INK4a Immunohistochemistry as a Biomarker for Cervical Precancer*
**作者**：Cuschieri K, Wentzensen N
**摘要**：该研究验证了p16INK4a抗体(克隆号E6H4)在宫颈活检组织中的过表达与高危型HPV感染的相关性，提出p16可作为宫颈癌前病变的辅助诊断标志物。
2. **文献名称**：*Utility of p16 Immunostaining in Differentiating Uterine Serous Carcinoma From Endometrioid Carcinoma*
**作者**：Alkhasawneh A, et al.
**摘要**：通过对比p16抗体(克隆号G175-405)在子宫浆液性癌和子宫内膜样癌中的表达差异，研究发现强弥漫性p16染色可作为鉴别这两种亚型的重要依据。
3. **文献名称**：*p16 Expression in Head and Neck Squamous Cell Carcinoma: Prognostic Significance and Correlation with HPV Status*
**作者**：Westra WH, et al.
**摘要**：利用p16抗体(克隆号JC8)检测头颈部鳞癌组织，发现p16过表达与HPV感染高度相关，且p16阳性患者预后显著优于阴性患者，支持其作为HPV相关肿瘤的生物标志物。
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**注**：上述文献中提到的"ARC抗体"可能指特定实验室使用的克隆号(如E6H4、JC8等)，实际引用时建议通过具体克隆号或DOI进一步确认文献准确性。
p16 (also known as CDKN2A or INK4a) is a tumor suppressor protein that regulates the cell cycle by inhibiting cyclin-dependent kinases (CDK4/6), thereby preventing uncontrolled cell proliferation. In the context of cervical pathology, p16 overexpression is strongly associated with high-risk human papillomavirus (HPV) infections, particularly HPV types 16 and 18. The viral oncoprotein E7 disrupts normal cell cycle control by inactivating retinoblastoma protein (pRb), leading to compensatory upregulation of p16 as part of a cellular stress response. This makes p16 a reliable biomarker for HPV-induced neoplastic transformation.
The p16 ARC (Antigen Retrieval Citrate) antibody is a monoclonal antibody (clone E6H4) optimized for immunohistochemical (IHC) detection of p16 in formalin-fixed, paraffin-embedded tissues. Its standardized use in cervical biopsies helps distinguish high-grade squamous intraepithelial lesions (HSIL) from benign reactive changes, improving diagnostic accuracy. The "ARC" designation refers to its validation for antigen retrieval using citrate buffer, a critical step for epitope exposure in IHC protocols. Beyond cervical cancer, p16 IHC with ARC-class antibodies aids in diagnosing HPV-related oropharyngeal cancers and other malignancies linked to cell cycle dysregulation. Its role in cervical screening complements HPV DNA testing and cytology, offering prognostic value for lesion progression.
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